| Proteomic analysis of the intestinal adaptation response reveals altered expression of fatty acid binding proteins following massive small bowel resection. | |
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MedLine Citation:
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PMID: 19943703 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intestinal adaptation in response to the loss of the small intestine is essential to restore enteral autonomy in patients who have undergone massive small bowel resection (MSBR). In a proportion of patients, intestinal function is not restored, resulting in chronic intestinal failure (IF). Early referral of such patients for transplant provides the best prognosis; however, the molecular mechanisms underlying intestinal adaptation remain elusive and there is currently no convenient marker to predict whether patients will develop IF. We have investigated the adaptation response in a well-characterized porcine model of intestinal adaptation. 2D DIGE analysis of ileal epithelium from piglets recovering from massive small bowel resection (MSBR) identified over 60 proteins that changed specifically in MSBR animals relative to nonoperational or sham-operated controls. Three fatty acid binding proteins (L-FABP, FABP-6, and I-FABP) showed changes in MSBR animals. The expression changes and localization of each FABP were validated by immunoblotting and immunohistochemical analysis. FABP expression changes in MSBR animals occurred concurrently with altered triglyceride and bile acid metabolism as well as weight gain. The observed FABP expression changes in the ileal epithelium occur as part of the intestinal adaptation response and could provide a clinically useful marker to evaluate adaptation following MSBR. |
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Authors:
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Andrew N Stephens; Prue M Pereira-Fantini; Guineva Wilson; Russell G Taylor; Adam Rainczuk; Katie L Meehan; Magdy Sourial; Peter J Fuller; Peter G Stanton; David M Robertson; Julie E Bines |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of proteome research Volume: 9 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-05 Completed Date: 2010-06-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 1437-49 Citation Subset: IM |
Affiliation:
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Prince Henry's Institute of Medical Research, Monash Medical Centre, Clayton, Victoria, Australia. Andrew.stephens@princehenrys.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Biological
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genetics,
physiology* Animals Cluster Analysis Electrophoresis, Gel, Two-Dimensional Fatty Acid-Binding Proteins / analysis, biosynthesis*, genetics Gene Expression Regulation Ileum / cytology, metabolism, physiology Intestinal Mucosa / metabolism* Intestine, Small / metabolism, physiology*, surgery* Proteomics / methods* Reproducibility of Results Short Bowel Syndrome / genetics, metabolism Signal Transduction Swine |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acid-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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