Document Detail


Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis.
MedLine Citation:
PMID:  22243521     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chronic pancreatitis is characterized by inflammation, fibrosis, pain, and loss of exocrine function of the pancreas. We aimed to identify differentially expressed proteins in the ePFT-collected pancreatic fluid from individuals with chronic pancreatitis (CP; n = 9) and controls with chronic abdominal pain not associated with the pancreas (NP; n = 9). Using GeLC-MS/MS techniques, we identified a total of 1391 different proteins in 18 pancreatic fluid samples. Of these proteins, 257 and 413 were identified exclusively in the control and chronic pancreatitis cohorts, respectively, and 721 were identified in both cohorts. Spectral counting and statistical analysis thereof revealed an additional 38 and 77 proteins that were up- or down-regulated, respectively, in the pancreatic fluid from individuals with chronic pancreatitis. As expected, gene ontology analysis illustrated that the largest percentage of differentially regulated proteins was secreted/extracellular in origin. In addition, proteins that were down-regulated with statistical significance in the chronic pancreatitis cohort were determined to have biological function of proteases, corresponding to the canonical pancreatic insufficiency associated with chronic pancreatitis. Proteins enriched in the pancreatic fluid of chronic pancreatitis patients had roles in fibrosis, inflammation, and pain, whereas digestive enzymes were significantly less abundant. Our workflow provided a mass spectrometry-based approach for the further study of the pancreatic fluid proteome, which may lead to the discovery potential biomarkers of chronic pancreatitis.
Authors:
Joao A Paulo; Vivek Kadiyala; Linda S Lee; Peter A Banks; Darwin L Conwell; Hanno Steen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-02-07
Journal Detail:
Title:  Journal of proteome research     Volume:  11     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-02     Completed Date:  2012-07-09     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1897-912     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / metabolism
Case-Control Studies
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Endoscopy, Gastrointestinal
Female
Gene Expression Regulation
Humans
Male
Metabolic Networks and Pathways
Middle Aged
Pancreas / metabolism*,  secretion
Pancreatic Juice / metabolism*
Pancreatitis, Chronic / metabolism*
Proteome / genetics,  metabolism*,  secretion
Proteomics
Tandem Mass Spectrometry
Young Adult
Grant Support
ID/Acronym/Agency:
1 P01 CA130821/CA/NCI NIH HHS; F32 DK085835/DK/NIDDK NIH HHS; F32 DK085835-02/DK/NIDDK NIH HHS; P30 CA51008/CA/NCI NIH HHS; P30 DK034854/DK/NIDDK NIH HHS; P30 DK034854-27/DK/NIDDK NIH HHS; R01CA129813/CA/NCI NIH HHS; R21 DK081703/DK/NIDDK NIH HHS; R21 DK081703-02/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Proteome
Comments/Corrections

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