Document Detail

Proteomic analysis of ethene-enriched groundwater microcosms from a vinyl chloride-contaminated site.
MedLine Citation:
PMID:  20121086     Owner:  NLM     Status:  MEDLINE    
Contamination of groundwater with vinyl chloride (VC), a known human carcinogen, is a common environmental problem at plastics manufacturing, dry cleaning, and military sites. At many sites, there is the potential to cleanup VC groundwater plumes with aerobic VC-oxidizing microorganisms (e.g., methanotrophs, etheneotrophs, and VC-assimilating bacteria). Environmental biotechnologies that reveal the presence and activity of VC-oxidizing bacteria in contaminated groundwater samples would provide valuable lines of evidence that bioremediation of VC is occurring at a site. We applied targeted shotgun mass spectrometry-based proteomic methods to ethene-enriched groundwater microcosms from a VC-contaminated site. Polypeptides from the enzymes alkene monooxygenase (EtnC) and epoxyalkane:CoM transferase (EtnE), both of which are expressed by aerobic etheneotrophs and VC-assimilating bacteria, were identified in 7 of the 14 samples analyzed. Bioinformatic analysis revealed that 2 EtnC and 5 EtnE peptides were unique to deduced EtnC and EtnE sequences from two different cultivated strains. In addition, several partial EtnE genes sequenced from microcosms matched with observed EtnE peptides. Our results have revealed broader etheneotroph functional gene diversity and demonstrate the feasibility, speed, and accuracy of applying a targeted metaproteomics approach to identifying protein biomarkers from etheneotrophs in complex environmental samples.
Adina S Chuang; Yang Oh Jin; Laura S Schmidt; Yalan Li; Samuel Fogel; Donna Smoler; Timothy E Mattes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Environmental science & technology     Volume:  44     ISSN:  0013-936X     ISO Abbreviation:  Environ. Sci. Technol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-25     Completed Date:  2010-04-29     Revised Date:  2012-07-13    
Medline Journal Info:
Nlm Unique ID:  0213155     Medline TA:  Environ Sci Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1594-601     Citation Subset:  IM    
Department of Civil and Environmental Engineering, University of Iowa, Iowa City, IA, USA.
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MeSH Terms
Bacteria / enzymology,  genetics*,  isolation & purification
Carbon-Sulfur Lyases / genetics*
Carcinogens / analysis
Ethylenes / analysis*
Genetic Variation
Oxygenases / genetics
Proteome / genetics*
Vinyl Chloride / analysis*,  toxicity
Water Microbiology
Water Pollutants, Chemical / analysis*
Reg. No./Substance:
0/Carcinogens; 0/Ethylenes; 0/Proteome; 0/Water Pollutants, Chemical; 74-85-1/ethylene; 75-01-4/Vinyl Chloride; EC 1.13.-/Oxygenases; EC 1.14.13.-/alkene monooxygenase; EC 4.4.-/Carbon-Sulfur Lyases; EC 2-mercaptoethanesulfonate lyase (epoxyalkane-ring forming)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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