| Proteomic Analysis of Colon Tissue from Interleukin-10 Gene-deficient Mice Fed Polyunsaturated Fatty Acids with Comparison to Transcriptomic Analysis. | |
| | |
MedLine Citation:
|
PMID: 22106967 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Inflammatory bowel disease (IBD) is characterized by intestinal inflammation and is believed to involve complex interactions between genetic, immunological, and environmental factors. We measured changes in the proteome associated with bacterially-induced intestinal inflammation in the interleukin 10 gene-deficient (<i>Il10<sup>-/-</sup></i>) mouse model of IBD, established effects of the dietary polyunsaturated fatty acids (PUFAs) n-3 eicosapentaenoic acid (EPA), and n-6 arachidonic acid (AA) on protein expression (using oleic acid as a control fatty acid), and compared these changes with previously observed transcriptome changes in the same model. Ingenuity Pathways Analysis of proteomics data showed bacterially-induced inflammation was associated with reduced expression of proteins from pathways of metabolism and digestion/absorption/excretion of nutrients/ions, and increased expression of cellular stress and immune response proteins. Both PUFA treatments showed anti-inflammatory activity; EPA appeared to act via the PPARα pathway, whereas AA appeared to increase energy metabolism and cytoskeletal organization, and reduce cellular stress responses, possibly enabling a more robust response to inflammation. While there was agreement between proteomic and transcriptomic data with respect to pathways, there was limited concordance between individual gene and protein data, reflecting the importance of having both gene and protein data to better understand complex diseases such as IBD. |
| | |
Authors:
|
Janine M Cooney; Matthew Philip Greig Barnett; Diane Brewster; Bianca Knoch; Warren C McNabb; William A Laing; Nicole C Roy |
Related Documents
:
|
2054387 - The nucleotide sequence of rabbit liver transferrin cdna. 18814897 - Molecular and structural characterization of hiv-1 subtype b brazilian isolates with gw... 6090927 - Mutations that alter the dna sequence specificity of the catabolite gene activator prot... 16862457 - Determination of primary structure of two isoforms 6-1 and 6-2 pla2 d49 from bothrops j... 19537927 - Regulation of diurnal variation of cholesterol 7alpha-hydroxylase (cyp7a1) activity in ... 7543897 - Characterization of the antigenic specificity of four different anti-(alpha 2-->8-linke... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-22 |
Journal Detail:
|
Title: Journal of proteome research Volume: - ISSN: 1535-3907 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Change in Cardiovascular Disease Status in Peritoneal Dialysis Patients: A 5-Year Single-Center Expe...
Next Document: Ortho-Lithium/Magnesium Carboxylate-Driven Aromatic Nucleophilic Substitution Reactions on Unprotect...