Document Detail


Proteomic analysis of survival of Rhodococcus jostii RHA1 during carbon starvation.
MedLine Citation:
PMID:  22798368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhodococcus jostii RHA1, a catabolically diverse soil actinomycete, is highly resistant to long-term nutrient starvation. After 2 years of carbon starvation, 10% of the bacterial culture remained viable. To study the molecular basis of such resistance, we monitored the abundance of about 1,600 cytosolic proteins during a 2-week period of carbon source (benzoate) starvation. Hierarchical cluster analysis elucidated 17 major protein clusters and showed that most changes occurred during transition to stationary phase. We identified 196 proteins. A decrease in benzoate catabolic enzymes correlated with benzoate depletion, as did induction of catabolism of alternative substrates, both endogenous (lipids, carbohydrates, and proteins) and exogenous. Thus, we detected a transient 5-fold abundance increase for phthalate, phthalate ester, biphenyl, and ethyl benzene catabolic enzymes, which coincided with at least 4-fold increases in phthalate and biphenyl catabolic activities. Stationary-phase cells demonstrated an ∼250-fold increase in carbon monoxide dehydrogenase (CODH) concurrent with a 130-fold increase in CODH activity, suggesting a switch to CO or CO(2) utilization. We observed two phases of stress response: an initial response occurred during the transition to stationary phase, and a second response occurred after the cells had attained stationary phase. Although SigG synthesis was induced during starvation, a ΔsigG deletion mutant showed only minor changes in cell survival. Stationary-phase cells underwent reductive cell division. The extreme capacity of RHA1 to survive starvation does not appear to involve novel mechanisms; rather, it seems to be due to the coordinated combination of earlier-described mechanisms.
Authors:
Marianna A Patrauchan; Daisuke Miyazawa; Justin C LeBlanc; Carol Aiga; Christine Florizone; Manisha Dosanjh; Julian Davies; Lindsay D Eltis; William W Mohn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-13
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-27     Completed Date:  2012-12-12     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6714-25     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis*
Carbon / metabolism*
Cytosol / chemistry
Proteome / analysis*
Rhodococcus / chemistry*,  metabolism,  physiology*
Stress, Physiological*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Proteome; 7440-44-0/Carbon
Comments/Corrections

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