Document Detail


Proteome profiling of flax (Linum usitatissimum) seed: Characterization of functional metabolic pathways operating during seed development.
MedLine Citation:
PMID:  23153172     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Flax (Linum usitatissimum L.) seeds are an important source of food and feed due to the presence of various health promoting compounds, making it a nutritionally and economically important plant. An in-depth analysis of the proteome of developing flax seed is expected to provide significant information with respect to the regulation and accumulation of such storage compounds. Therefore, a proteomic analysis of seven seed developmental stages (4, 8, 12, 16, 22, 30 and 48 days after anthesis) in a flax variety, NL-97 was carried out using a combination of 1D-SDS-PAGE and LC-MS(E) methods. A total 1716 proteins were identified and their functional annotation revealed that a majority of them were involved in primary metabolism, protein destination, storage and energy. Three carbon assimilatory pathways appeared to operate in flax seeds. Reverse transcription quantitative PCR of selected 19 was carried out to understand their role during seed development. Besides storage proteins, methionine synthase, RuBisCO and S-adenosylmethionine synthetase, were highly expressed transcripts, highlighting their importance in flax seed development. Further, the identified proteins were mapped on to developmental seed specific EST libraries of flax to obtain transcriptional evidence and 81% of them had detectable expression at mRNA level. This study provides new insights into the complex seed developmental processes operating in flax.
Authors:
Vitthal T Barvkar; Varsha C Pardeshi; Sandip M Kale; Narendra Y Kadoo; Ashok P Giri; Vidya S Gupta
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-16
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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