Document Detail

Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7.
MedLine Citation:
PMID:  19533175     Owner:  NLM     Status:  MEDLINE    
Using two-dimensional gel electrophoresis (2-DE) and mass spectrometry, the proteome of a metabolically engineered succinic acid-overproducing bacterium, Mannheimia succiniciproducens LPK7, was examined and compared with that of its wild type strain, MBEL55E, to elucidate the physiological and metabolic changes responsible for succinic acid overproduction and cell growth. Comparative proteomic studies clearly showed that the expression levels of enzymes involved in the ATP formation and consumption (AtpD, Ppa, SerS, ProS, Pnp, PotD, MalK, RbsB, and TbpA), pyruvate metabolism (AceF and Lpd), glycolysis (GapA, Pgk, Fba, and TpiA), and amino acid biosynthesis (Asd, DapA, DapD, Gdh, ArgD, and ArgG) varied significantly in the LPK7 strain compared with those in the MBEL55E strain. Based on the comparative proteome profiling, the formation of pyruvic acid, a newly formed byproduct in the engineered LPK7 strain, could be reduced by adding into the culture medium pantothenate and L: -cysteine, which serve as precursors of CoA biosynthesis.
Jeong Wook Lee; Sang Yup Lee
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-17
Journal Detail:
Title:  Bioprocess and biosystems engineering     Volume:  33     ISSN:  1615-7605     ISO Abbreviation:  Bioprocess Biosyst Eng     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-21     Completed Date:  2010-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088505     Medline TA:  Bioprocess Biosyst Eng     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  97-107     Citation Subset:  IM    
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
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MeSH Terms
Bacterial Proteins / biosynthesis*,  genetics
Cysteine / pharmacology
Enzymes / biosynthesis*,  genetics
Gene Expression Regulation, Bacterial / drug effects,  genetics
Gene Expression Regulation, Enzymologic / drug effects,  genetics
Genetic Engineering / methods
Mannheimia / enzymology*,  genetics,  growth & development
Pantothenic Acid / pharmacology
Proteome / biosynthesis*,  genetics
Pyruvic Acid / metabolism
Species Specificity
Succinic Acid / metabolism*
Vitamin B Complex / pharmacology
Reg. No./Substance:
0/Bacterial Proteins; 0/Enzymes; 0/Proteome; 110-15-6/Succinic Acid; 12001-76-2/Vitamin B Complex; 127-17-3/Pyruvic Acid; 52-90-4/Cysteine; 79-83-4/Pantothenic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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