Document Detail


Proteome analysis reveals a distinct molecular profile of cellular stress following incubation of DDT1-MF2 smooth muscle cells in the presence of a high concentration of hyperforin.
MedLine Citation:
PMID:  15095150     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The acylphloroglucinol derivative hyperforin is a major constituent of St. John's wort extracts ( Hypericum perforatum L.), which has been demonstrated to contribute to the antidepressant action of this herbal drug. In previous investigations we observed that hyperforin causes a rapid stimulation of intracellular calcium mobilization and enhances extracellular acidification in the hamster vas deferens smooth muscle cell line DDT (1)-MF2. To obtain further insight into its mode of action, we have now examined if these effects are accompanied by changes in protein expression. Cells were incubated with hyperforin for 15 min at a concentration of 1 microg/mL. Proteome analysis in cell lysates was accomplished by two-dimensional gel electrophoresis (2D-PAGE) and proteins were visualized by silver staining. Differences in the expression pattern between hyperforin- and vehicle-treated cells were displayed by computer-assisted differential display and identification of selected protein spots was performed by peptide mass fingerprinting after digestion with trypsin. Following incubation with hyperforin marked changes in the expression of several proteins were evident. A particularly strong change was observed for 6 proteins, which were identified as tubulin-beta, enolase 3, SYNCRIP, endoplasmin, elongation factor 2 and HSP84. As these proteins are known to be involved in cellular responses to stress by regulating energy metabolism as well as synthesis, intracellular transport and folding of proteins, our results suggest that the effects observed are not components of the normal pharmacological activity profile of hyperforin but are rather indicative of cellular stress promoting activity at higher concentrations.
Authors:
André Schrattenholz; Klaus Schroer; Shyam S Chatterjee; Egon Koch
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  70     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-19     Completed Date:  2004-06-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  342-6     Citation Subset:  IM    
Affiliation:
Proteosys AG, Mainz, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antidepressive Agents / administration & dosage,  pharmacology*,  therapeutic use
Bicyclo Compounds
Cells, Cultured / drug effects
Cricetinae
Electrophoresis, Gel, Two-Dimensional
Hypericum*
Male
Mesocricetus
Muscle, Smooth / drug effects*,  metabolism
Phloroglucinol / analogs & derivatives
Phytotherapy*
Plant Extracts / administration & dosage,  pharmacology,  therapeutic use
Proteome / drug effects*
Stress, Physiological / prevention & control
Terpenes / administration & dosage,  pharmacology*,  therapeutic use
Vas Deferens / cytology
Chemical
Reg. No./Substance:
0/Antidepressive Agents; 0/Bicyclo Compounds; 0/Plant Extracts; 0/Proteome; 0/Terpenes; 108-73-6/Phloroglucinol; 11079-53-1/hyperforin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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