Document Detail

Proteome analysis of human placentae: pre-eclampsia versus normal pregnancy.
MedLine Citation:
PMID:  17182098     Owner:  NLM     Status:  MEDLINE    
Although placental proteins play multiple roles in fetal and placental development and in the maintenance of pregnancy, many remain inadequately characterized. In the present study, we comprehensively analyzed these proteins by using a proteomic approach. Samples were denatured with guanidine hydrochloride, which was found to be superior to the commonly used urea for the present purpose, and subjected to 2-dimensional (2D) electrophoresis (2-DE) to obtain placental proteome maps. The identified protein spots (ca. 60% of the total) on the proteome maps included several pregnancy-related proteins (PRPs). Furthermore, a novel 2D immunoblotting (2-DI) analysis of molecules related to pre-eclampsia revealed three immunopositive spots that appeared to correspond to dynactin p-50, a protein related to cell turn-over. The rate of positivity for dynactin p-50-reactive antibodies was significantly (P=0.0024) higher in 26 pre-eclamptic women than in 58 normally pregnant women. These results indicate that dynactin p-50 may be involved in the pathophysiology of pre-eclampsia.
K Mine; A Katayama; T Matsumura; T Nishino; Y Kuwabara; G Ishikawa; T Murata; R Sawa; Y Otsubo; S Shin; T Takeshita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-19
Journal Detail:
Title:  Placenta     Volume:  28     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-11     Completed Date:  2007-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  676-87     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
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MeSH Terms
Antibodies / immunology
Electrophoresis, Gel, Two-Dimensional
Guanidine / chemistry
Microtubule-Associated Proteins / analysis*,  blood
Placenta / chemistry*,  metabolism
Pre-Eclampsia / metabolism*
Pregnancy / metabolism*
Protein Denaturation
Proteome / analysis*
Reg. No./Substance:
0/Antibodies; 0/Microtubule-Associated Proteins; 0/Proteome; 113-00-8/Guanidine; 144198-36-7/dynactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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