Document Detail


Proteome analysis of human amnion and amniotic fluid by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
MedLine Citation:
PMID:  16294308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteome analysis by 2-DE and PMF by MALDI-TOF MS was performed on human amnion and amniotic fluid at term. Ninety-two soluble and nineteen membrane proteins were identified from amnion. Thirty-five proteins were identified from amniotic fluid. Calgranulin A and B were found in all patients infected with Ureaplasma urealyticum, but not in any of the patients without infection, indicating that they are potential markers of intrauterine infection. Identity of calgranulin A and B was confirmed by MALDI-TOF/TOF MS. This study represents the first extensive analysis of the human amnion and amniotic fluid proteome at term and demonstrates that 2-DE and MALDI-TOF MS is a useful tool for identifying clinically significant biomarkers of problematic pregnancies.
Authors:
Soo-Jin Park; Won-Gap Yoon; Jin-Su Song; Hyun Sook Jung; Chong Jai Kim; Soo Young Oh; Bo Hyun Yoon; Guhung Jung; Hie-Joon Kim; Takashi Nirasawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  6     ISSN:  1615-9853     ISO Abbreviation:  Proteomics     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-03-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  349-63     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Seoul National University, Gwanak-gu, Seoul, Korea 151-747.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amnion / chemistry*
Amniotic Fluid / chemistry*
Base Sequence
Calgranulin A / isolation & purification
Calgranulin B / chemistry,  isolation & purification
DNA Primers
Electrophoresis, Gel, Two-Dimensional / methods*
Humans
Molecular Sequence Data
Proteome*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Ureaplasma Infections / metabolism
Chemical
Reg. No./Substance:
0/Calgranulin A; 0/Calgranulin B; 0/DNA Primers; 0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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