Document Detail


Proteome analysis of adipocyte lipid rafts reveals that gC1qR plays essential roles in adipogenesis and insulin signal transduction.
MedLine Citation:
PMID:  19402044     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since insulin receptors and their downstream signaling molecules are organized in lipid rafts, proteomic analysis of adipocyte lipid rafts may provide new insights into the function of lipid rafts in adipogenesis and insulin signaling. To search for proteins involved in adipocyte differentiation and insulin signaling, we analyzed detergent-resistant lipid raft proteins from 3T3-L1 preadipocytes and adipocytes by 2-DE. Eleven raft proteins were identified from adipocytes. One of the adipocyte-specific proteins was globular C1q receptor (gC1qR), an acidic 32 kDa protein known as the receptor for the globular domain of complement C1q. The targeting of gC1qR into lipid rafts was significantly increased during adipogenesis, as determined by immunoblotting and immunofluorescence. Since the silencing of gC1qR by small RNA interference abolished adipogenesis and blocked insulin-induced activation of insulin receptor, insulin receptor substrate-1 (IRS-1), Akt, and Erk1/2, we can conclude that gC1qR is an essential molecule involved in adipogenesis and insulin signaling.
Authors:
Ki-Bum Kim; Bong-Woo Kim; Hyo-Jung Choo; Young-Chan Kwon; Byung-Yoon Ahn; Jong-Soon Choi; Jae-Seon Lee; Young-Gyu Ko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  9     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2373-82     Citation Subset:  IM    
Affiliation:
College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / chemistry*,  metabolism
Adipogenesis*
Amino Acid Sequence
Animals
Antigens, CD44 / analysis,  metabolism*
Cell Line
Down-Regulation
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation
Insulin / metabolism*
Membrane Microdomains / chemistry*,  metabolism
Mice
Molecular Sequence Data
Proteome / analysis*
RNA Interference
Signal Transduction
Chemical
Reg. No./Substance:
0/Antigens, CD44; 0/C1qbp protein, mouse; 0/Proteome; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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