Document Detail


Proteome analysis of Streptococcus mutans metabolic phenotype during acid tolerance.
MedLine Citation:
PMID:  15133097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two-dimensional gel electrophoretic analysis of the proteome of Streptococcus mutans grown at a steady state in a glucose-limited anaerobic continuous culture revealed a number of proteins that were differentially expressed when the growth pH was lowered from pH 7.0 to pH 5.0. Changes in the expression of metabolic proteins were generally limited to three biochemical pathways: glycolysis, alternative acid production and branched-chain amino acid biosynthesis. The relative level of expression of protein spots representing all of the enzymes associated with the Embden-Meyerhof-Parnas pathway, and all but one of the enzymes involved in the major alternative acid fermentation pathways of S. mutans, was identified and measured. Proteome data, in conjunction with end-product and cell-yield analyses, were consistent with a phenotypic change that allowed S. mutans to proliferate at low pH by expending energy to extrude excess H(+) from the cell, while minimizing the detrimental effects that result from the uncoupling of carbon flux from catabolism and the consequent imbalance in NADH and pyruvate production. The changes in enzyme levels were consistent with a reduction in the formation of the strongest acid, formic acid, which was a consequence of the diversion of pyruvate to both lactate and branched-chain amino acid production when S. mutans was cultivated in an acidic environment.
Authors:
Alice C L Len; Derek W S Harty; Nicholas A Jacques
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  150     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-10     Completed Date:  2004-07-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  1353-66     Citation Subset:  IM    
Affiliation:
Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, PO Box 533, Wentworthville, NSW, Australia 2145.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids, Branched-Chain / metabolism
Anaerobiosis
Bacterial Proteins / genetics,  metabolism*
Culture Media
Electrophoresis, Gel, Two-Dimensional
Formic Acids / metabolism
Gene Expression Regulation, Bacterial*
Glucose / metabolism
Glycolysis
Hydrogen-Ion Concentration
Phenotype
Proteome*
Streptococcus mutans / genetics,  growth & development*,  metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
R01 DE 013234/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids, Branched-Chain; 0/Bacterial Proteins; 0/Culture Media; 0/Formic Acids; 0/Proteome; 50-99-7/Glucose; 64-18-6/formic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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