Document Detail

Proteome analysis of Halobacterium sp. NRC-1 facilitated by the biomodule analysis tool BMSorter.
MedLine Citation:
PMID:  16497792     Owner:  NLM     Status:  MEDLINE    
To better understand the extremely halophilic archaeon Halobacterium species NRC-1, we analyzed its soluble proteome by two-dimensional liquid chromatography coupled to electrospray ionization tandem mass spectrometry. A total of 888 unique proteins were identified with a ProteinProphet probability (P) between 0.9 and 1.0. To evaluate the biochemical activities of the organism, the proteomic data were subjected to a biological network analysis using our BMSorter software. This allowed us to examine the proteins expressed in different biomodules and study the interactions between pertinent biomodules. Interestingly an integrated analysis of the enzymes in the amino acid metabolism and citrate cycle networks suggested that up to eight amino acids may be converted to oxaloacetate, fumarate, or oxoglutarate in the citrate cycle for energy production. In addition, glutamate and aspartate may be interconverted from other amino acids or synthesized from citrate cycle intermediates to meet the high demand for the acidic amino acids that are required to build the highly acidic proteome of the organism. Thus this study demonstrated that proteome analysis can provide useful information and help systems analyses of organisms.
Rueichi R Gan; Eugene C Yi; Yulun Chiu; Hookeun Lee; Yu-Chieh P Kao; Timothy H Wu; Ruedi Aebersold; David R Goodlett; Wailap Victor Ng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-23
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  5     ISSN:  1535-9476     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-15     Completed Date:  2006-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  987-97     Citation Subset:  IM    
Institute of Biochemistry, National Yang Ming University, Taipei City Hospital, Taipei 112, Taiwan, Republic of China.
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MeSH Terms
Amino Acids / metabolism
Archaeal Proteins / analysis*,  genetics,  metabolism
Biological Transport
Chromatography, Liquid
Computational Biology / methods
Genetic Processes
Genome, Archaeal
Halobacterium / chemistry*,  genetics,  metabolism*
Mass Spectrometry
Proteome / analysis*
Reg. No./Substance:
0/Amino Acids; 0/Archaeal Proteins; 0/Proteome

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