| Proteome analysis of Halobacterium sp. NRC-1 facilitated by the biomodule analysis tool BMSorter. | |
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MedLine Citation:
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PMID: 16497792 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To better understand the extremely halophilic archaeon Halobacterium species NRC-1, we analyzed its soluble proteome by two-dimensional liquid chromatography coupled to electrospray ionization tandem mass spectrometry. A total of 888 unique proteins were identified with a ProteinProphet probability (P) between 0.9 and 1.0. To evaluate the biochemical activities of the organism, the proteomic data were subjected to a biological network analysis using our BMSorter software. This allowed us to examine the proteins expressed in different biomodules and study the interactions between pertinent biomodules. Interestingly an integrated analysis of the enzymes in the amino acid metabolism and citrate cycle networks suggested that up to eight amino acids may be converted to oxaloacetate, fumarate, or oxoglutarate in the citrate cycle for energy production. In addition, glutamate and aspartate may be interconverted from other amino acids or synthesized from citrate cycle intermediates to meet the high demand for the acidic amino acids that are required to build the highly acidic proteome of the organism. Thus this study demonstrated that proteome analysis can provide useful information and help systems analyses of organisms. |
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Authors:
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Rueichi R Gan; Eugene C Yi; Yulun Chiu; Hookeun Lee; Yu-Chieh P Kao; Timothy H Wu; Ruedi Aebersold; David R Goodlett; Wailap Victor Ng |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-23 |
Journal Detail:
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Title: Molecular & cellular proteomics : MCP Volume: 5 ISSN: 1535-9476 ISO Abbreviation: Mol. Cell Proteomics Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-15 Completed Date: 2006-07-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101125647 Medline TA: Mol Cell Proteomics Country: United States |
Other Details:
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Languages: eng Pagination: 987-97 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, National Yang Ming University, Taipei City Hospital, Taipei 112, Taiwan, Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Archaeal Proteins / analysis*, genetics, metabolism Biological Transport Chromatography, Liquid Computational Biology / methods Genetic Processes Genome, Archaeal Halobacterium / chemistry*, genetics, metabolism* Mass Spectrometry Proteome / analysis* Software |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Archaeal Proteins; 0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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