Document Detail


Proteome of human subcutaneous adipose tissue stromal vascular fraction cells versus mature adipocytes based on DIGE.
MedLine Citation:
PMID:  21261302     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adipose tissue contains a heterogeneous population of mature adipocytes, endothelial cells, immune cells, pericytes, and preadipocytic stromal/stem cells. To date, a majority of proteomic analyses have focused on intact adipose tissue or isolated adipose stromal/stem cells in vitro. In this study, human subcutaneous adipose tissue from multiple depots (arm and abdomen) obtained from female donors was separated into populations of stromal vascular fraction cells and mature adipocytes. Out of 960 features detected by 2-D gel electrophoresis, a total of 200 features displayed a 2-fold up- or down-regulation relative to each cell population. The protein identity of 136 features was determined. Immunoblot analyses comparing SVF relative to adipocytes confirmed that carbonic anhydrase II was up-regulated in both adipose depots while catalase was up-regulated in the arm only. Bioinformatic analyses of the data set determined that cytoskeletal, glycogenic, glycolytic, lipid metabolic, and oxidative stress related pathways were highly represented as differentially regulated between the mature adipocytes and stromal vascular fraction cells. These findings extend previous reports in the literature with respect to the adipose tissue proteome and the consequences of adipogenesis. The proteins identified may have value as biomarkers for monitoring the physiology and pathology of cell populations within subcutaneous adipose depots.
Authors:
Indu Kheterpal; Ginger Ku; Liana Coleman; Gang Yu; Andrey A Ptitsyn; Z Elizabeth Floyd; Jeffrey M Gimble
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-03-04
Journal Detail:
Title:  Journal of proteome research     Volume:  10     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-01     Completed Date:  2011-09-16     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1519-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / chemistry*
Adult
Carbonic Anhydrase II / metabolism
Cell Differentiation
Cells, Cultured
Computational Biology / methods
Electrophoresis, Gel, Two-Dimensional / methods*
Endothelial Cells / chemistry*,  cytology
Female
Humans
Mass Spectrometry / methods
Middle Aged
Phenotype
Proteome / analysis*
Stem Cells / chemistry,  cytology
Stromal Cells / chemistry*,  cytology
Subcutaneous Fat / cytology*
Grant Support
ID/Acronym/Agency:
P20 GM103528/GM/NIGMS NIH HHS; P20 RR021945/RR/NCRR NIH HHS; P20 RR021945-05/RR/NCRR NIH HHS; P20-RR021945/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Proteome; EC 4.2.1.-/Carbonic Anhydrase II
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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