| Proteome of human subcutaneous adipose tissue stromal vascular fraction cells versus mature adipocytes based on DIGE. | |
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MedLine Citation:
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PMID: 21261302 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adipose tissue contains a heterogeneous population of mature adipocytes, endothelial cells, immune cells, pericytes, and preadipocytic stromal/stem cells. To date, a majority of proteomic analyses have focused on intact adipose tissue or isolated adipose stromal/stem cells in vitro. In this study, human subcutaneous adipose tissue from multiple depots (arm and abdomen) obtained from female donors was separated into populations of stromal vascular fraction cells and mature adipocytes. Out of 960 features detected by 2-D gel electrophoresis, a total of 200 features displayed a 2-fold up- or down-regulation relative to each cell population. The protein identity of 136 features was determined. Immunoblot analyses comparing SVF relative to adipocytes confirmed that carbonic anhydrase II was up-regulated in both adipose depots while catalase was up-regulated in the arm only. Bioinformatic analyses of the data set determined that cytoskeletal, glycogenic, glycolytic, lipid metabolic, and oxidative stress related pathways were highly represented as differentially regulated between the mature adipocytes and stromal vascular fraction cells. These findings extend previous reports in the literature with respect to the adipose tissue proteome and the consequences of adipogenesis. The proteins identified may have value as biomarkers for monitoring the physiology and pathology of cell populations within subcutaneous adipose depots. |
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Authors:
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Indu Kheterpal; Ginger Ku; Liana Coleman; Gang Yu; Andrey A Ptitsyn; Z Elizabeth Floyd; Jeffrey M Gimble |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-03-04 |
Journal Detail:
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Title: Journal of proteome research Volume: 10 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-01 Completed Date: 2011-09-16 Revised Date: 2012-09-19 |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 1519-27 Citation Subset: IM |
Affiliation:
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Proteomics and Metabolomics Core Facility, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, Louisiana 70808, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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chemistry* Adult Carbonic Anhydrase II / metabolism Cell Differentiation Cells, Cultured Computational Biology / methods Electrophoresis, Gel, Two-Dimensional / methods* Endothelial Cells / chemistry*, cytology Female Humans Mass Spectrometry / methods Middle Aged Phenotype Proteome / analysis* Stem Cells / chemistry, cytology Stromal Cells / chemistry*, cytology Subcutaneous Fat / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR021945-05/RR/NCRR NIH HHS; P20-RR021945/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteome; EC 4.2.1.-/Carbonic Anhydrase II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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