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Proteome Analysis of Distinct Developmental Stagesof Human Natural Killer Cells.
MedLine Citation:
PMID:  23315794     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The recent Natural Killer (NK) cell maturation model postulates that CD34+ hematopoietic stem cells (HSC) first develop into CD56bright NK cells, then into CD56dimCD57- and finally into terminally maturated CD56dimCD57+. The molecular mechanisms of human NK cell differentiation and maturation however are incompletely characterized. Here we present a proteome analysis of distinct developmental stages of human primary NK cells, isolated from healthy human blood donors. Peptide sequencing was used to comparatively analyze CD56bright NK cells versus CD56dim NK cells and CD56dimCD57-NK cells versus CD56dimCD57+ NK cells and revealed distinct protein signatures for all of these subsets. Quantitative data for about 3400 proteins were obtained and support the current differentiation model. Furthermore, 11 donor-independently, but developmental stage specifically regulated proteins so far un-described in NK cells were revealed, which may contribute to NK cell development and may elucidate a molecular source for NK cell effector functions. Among those proteins, S100A4 (Calvasculin) and S100A6 (Calcyclin) were selected to study their dynamic sub-cellular localization. Upon activation of human primary NK cells, both proteins are recruited into the immune synapse (NKIS), where they co-localize with Myosin IIa.
Authors:
Maxi Scheiter; Ulrike Lau; Marco van Ham; Bjoern Bulitta; Lothar Groebe; Henk Garritsen; Frank Klawonn; Sebastian Koenig; Lothar Jaensch
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-13
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  -     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Helmholtz Centre for Infection Research, Germany;
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