| Proteome Analysis of Distinct Developmental Stagesof Human Natural Killer Cells. | |
| | |
MedLine Citation:
|
PMID: 23315794 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The recent Natural Killer (NK) cell maturation model postulates that CD34+ hematopoietic stem cells (HSC) first develop into CD56bright NK cells, then into CD56dimCD57- and finally into terminally maturated CD56dimCD57+. The molecular mechanisms of human NK cell differentiation and maturation however are incompletely characterized. Here we present a proteome analysis of distinct developmental stages of human primary NK cells, isolated from healthy human blood donors. Peptide sequencing was used to comparatively analyze CD56bright NK cells versus CD56dim NK cells and CD56dimCD57-NK cells versus CD56dimCD57+ NK cells and revealed distinct protein signatures for all of these subsets. Quantitative data for about 3400 proteins were obtained and support the current differentiation model. Furthermore, 11 donor-independently, but developmental stage specifically regulated proteins so far un-described in NK cells were revealed, which may contribute to NK cell development and may elucidate a molecular source for NK cell effector functions. Among those proteins, S100A4 (Calvasculin) and S100A6 (Calcyclin) were selected to study their dynamic sub-cellular localization. Upon activation of human primary NK cells, both proteins are recruited into the immune synapse (NKIS), where they co-localize with Myosin IIa. |
| | |
Authors:
|
Maxi Scheiter; Ulrike Lau; Marco van Ham; Bjoern Bulitta; Lothar Groebe; Henk Garritsen; Frank Klawonn; Sebastian Koenig; Lothar Jaensch |
Related Documents
:
|
23554144 - Winner for outstanding research in the ph.d. category for the 2013 society for biomater... 23049564 - Burkitt lymphoma presenting as unilateral deafness in an immunocompetent patient. 23296934 - Mrna delivery to human dendritic cells by recombinant yeast and activation of antigen-s... 23103164 - A small molecule that promotes cardiac differentiation of human pluripotent stem cells ... 19534664 - Progenitor cell types in hiv-1 infection: bioactivity and emerging targets for treatment. 17141064 - Pluripotent stem cells from germ cells. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-13 |
Journal Detail:
|
Title: Molecular & cellular proteomics : MCP Volume: - ISSN: 1535-9484 ISO Abbreviation: Mol. Cell Proteomics Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101125647 Medline TA: Mol Cell Proteomics Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Helmholtz Centre for Infection Research, Germany; |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Proteomic analysis of the lung in rats with hypobaric hypoxia-induced pulmonary hypertension.
Next Document: Cadmium Induces Thymocyte Apoptosis via Caspase-Dependent and Caspase-Independent Pathways.