Document Detail


Proteome analysis of distinct developmental stages of human natural killer (NK) cells.
MedLine Citation:
PMID:  23315794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The recent Natural Killer (NK) cell maturation model postulates that CD34(+) hematopoietic stem cells (HSC) first develop into CD56(bright) NK cells, then into CD56(dim)CD57(-) and finally into terminally maturated CD56(dim)CD57(+). The molecular mechanisms of human NK cell differentiation and maturation however are incompletely characterized. Here we present a proteome analysis of distinct developmental stages of human primary NK cells, isolated from healthy human blood donors. Peptide sequencing was used to comparatively analyze CD56(bright) NK cells versus CD56(dim) NK cells and CD56(dim)CD57(-) NK cells versus CD56(dim)CD57(+) NK cells and revealed distinct protein signatures for all of these subsets. Quantitative data for about 3400 proteins were obtained and support the current differentiation model. Furthermore, 11 donor-independently, but developmental stage specifically regulated proteins so far undescribed in NK cells were revealed, which may contribute to NK cell development and may elucidate a molecular source for NK cell effector functions. Among those proteins, S100A4 (Calvasculin) and S100A6 (Calcyclin) were selected to study their dynamic subcellular localization. Upon activation of human primary NK cells, both proteins are recruited into the immune synapse (NKIS), where they colocalize with myosin IIa.
Authors:
Maxi Scheiter; Ulrike Lau; Marco van Ham; Björn Bulitta; Lothar Gröbe; Henk Garritsen; Frank Klawonn; Sebastian König; Lothar Jänsch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-13
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  12     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-07     Completed Date:  2013-12-09     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1099-114     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Antigens, CD56 / metabolism
Antigens, CD57 / metabolism
Cell Cycle Proteins / chemistry,  metabolism
Cell Differentiation
Cell Separation
Cells, Cultured
Humans
Immunological Synapses / metabolism
Killer Cells, Natural / physiology*
Lymphocyte Activation
Molecular Sequence Annotation
Molecular Sequence Data
Nonmuscle Myosin Type IIA / metabolism
Protein Transport
Proteome / chemistry,  metabolism*
S100 Proteins / chemistry,  metabolism
Signal Transduction
Chemical
Reg. No./Substance:
0/Antigens, CD56; 0/Antigens, CD57; 0/Cell Cycle Proteins; 0/NCAM1 protein, human; 0/Proteome; 0/S100 Proteins; 105504-00-5/S100A6 protein, human; 142662-27-9/S100A4 protein, human; EC 3.6.1.-/Nonmuscle Myosin Type IIA
Comments/Corrections

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