Document Detail


Proteome analysis of Cry4Ba toxin-interacting Aedes aegypti lipid rafts using geLC-MS/MS.
MedLine Citation:
PMID:  23153095     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid rafts are microdomains in the plasma membrane of eukaryotic cells. Among their many functions, lipid rafts are involved in cell toxicity caused by pore forming bacterial toxins including Bacillus thuringiensis (Bt) Cry toxins. We isolated lipid rafts from brush border membrane vesicles (BBMV) of Aedes aegypti larvae as a detergent resistant membrane (DRM) fraction on density gradients. Cholesterol, aminopeptidase (APN), alkaline phosphatase (ALP) and the raft marker flotillin were preferentially partitioned into the lipid raft fraction. When mosquitocidal Cry4Ba toxin was preincubated with BBMV, Cry4Ba localized to lipid rafts. A proteomic approach based on one-dimensional gel electrophoresis, in-gel trypsin digestion, followed by liquid chromatography-mass spectrometry (geLC-MS/MS) identified a total of 386 proteins. Of which many are typical lipid raft marker proteins including flotillins and glycosylphosphatidylinositol (GPI)-anchored proteins. Identified raft proteins were annotated in silico for functional and physicochemical characteristics. Parameters such as distribution of isoelectric point, molecular mass, and predicted post-translational modifications relevant to lipid raft proteins (GPI anchorage and myristoylation or palmitoylation) were analyzed for identified proteins in the DRM fraction. From a functional point of view, this study identified proteins implicated in Cry toxin interactions as well as membrane-associated proteins expressed in the mosquito midgut that have potential relevance to mosquito biology and vector management.
Authors:
Krishnareddy Bayyareddy; Xiang Zhu; Ron Orlando; Michael J Adang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-27
Journal Detail:
Title:  Journal of proteome research     Volume:  11     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-23     Revised Date:  2013-12-12    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5843-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aedes / chemistry*,  enzymology
Alkaline Phosphatase / chemistry
Aminopeptidases / chemistry
Animals
Bacterial Proteins / chemistry*
Chromatography, Liquid / methods*
Detergents
Electrophoresis, Polyacrylamide Gel
Endotoxins / chemistry*
Escherichia coli / chemistry
Glycosylphosphatidylinositols / chemistry
Hemolysin Proteins / chemistry*
Isoelectric Point
Larva / chemistry,  enzymology
Membrane Microdomains / chemistry*
Membrane Proteins / chemistry
Microvilli / chemistry
Octoxynol
Protein Interaction Mapping
Protein Processing, Post-Translational
Proteome / analysis*,  chemistry
Proteomics / methods
Receptors, Cell Surface / chemistry
Solubility
Tandem Mass Spectrometry / methods
Grant Support
ID/Acronym/Agency:
R01 AI 29092/AI/NIAID NIH HHS; R01 AI029092/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Detergents; 0/Endotoxins; 0/Glycosylphosphatidylinositols; 0/Hemolysin Proteins; 0/Membrane Proteins; 0/Proteome; 0/Receptors, Cell Surface; 0/flotillins; 0/insecticidal crystal protein, Bacillus Thuringiensis; 9002-93-1/Octoxynol; EC 3.1.3.1/Alkaline Phosphatase; EC 3.4.11.-/Aminopeptidases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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