Document Detail


Proteome alterations in primary human alveolar macrophages in response to influenza A virus infection.
MedLine Citation:
PMID:  22709384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To obtain a global picture of how alveolar macrophages respond to influenza A virus (IAV) infection, we used a quantitative proteomics method to systematically examine protein expression in the IAV-infected primary human alveolar macrophages. Of the 1214 proteins identified, 43 were significantly up-regulated and 63 significantly down-regulated at >95% confidence. The expression of an array of interferon (IFN)-induced proteins was significantly increased in the IAV-infected macrophages. The protein with the greatest expression increase was ISG15, an IFN-induced protein that has been shown to play an important role in antiviral defense. Concomitantly, quantitative real-time PCR analysis revealed that the gene expression of type I IFNs increased substantially following virus infection. Our results are consistent with the notion that type I IFNs play a vital role in the response of human alveolar macrophages to IAV infection. In addition to the IFN-mediated responses, inflammatory response, apoptosis, and redox state rebalancing appeared also to be major pathways that were affected by IAV infection. Furthermore, our data suggest that alveolar macrophages may play a crucial role in regenerating alveolar epithelium during IAV infection.
Authors:
Lin Liu; Jianhong Zhou; Yimeng Wang; Robert J Mason; Cornelius Joel Funk; Yuchun Du
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-05
Journal Detail:
Title:  Journal of proteome research     Volume:  11     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-06     Completed Date:  2012-12-05     Revised Date:  2013-08-13    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4091-101     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas 72701, United States.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Gene Expression
Gene Expression Regulation
Host-Pathogen Interactions
Humans
Immunity, Innate
Influenza A Virus, H1N1 Subtype / immunology,  physiology*
Influenza, Human / immunology,  metabolism*
Interferon Type I / metabolism
Macrophages, Alveolar / immunology,  metabolism*,  virology
Primary Cell Culture
Protein Interaction Maps
Proteome / genetics,  metabolism*
Viral Proteins / metabolism
Grant Support
ID/Acronym/Agency:
3P20RR015569-10S2/RR/NCRR NIH HHS; 5P20RR016460-11/RR/NCRR NIH HHS; 8 P20 GM103429-11/GM/NIGMS NIH HHS; P20 RR015569/RR/NCRR NIH HHS; P30 GM103450/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Interferon Type I; 0/Proteome; 0/Viral Proteins
Comments/Corrections

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