| Proteolytic processing of reovirus is required for adherence to intestinal M cells. | |
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MedLine Citation:
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PMID: 7525989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reovirus adheres specifically to apical membranes of mouse intestinal M cells and exploits M-cell transepithelial transport activity to enter Peyer's patch mucosa, where replication occurs. Proteolytic conversion of native reovirus to intermediate subviral particles (ISVPs) occurs in the intestine, but it is not known whether conversion is essential for interaction of virus with M cells. We tested the capacity of native virions, ISVPs, and cores (that lack outer capsid proteins) to bind to intestinal epithelial cells in vivo and found that only ISVPs adhered to M cells. Thus, intraluminal conversion of native reovirus to ISVPs is a prerequisite for M-cell adherence, and outer capsid proteins unique to ISVPs (either sigma 1 or products of mu 1) mediate interaction of virus with M-cell apical membranes. |
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Authors:
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H M Amerongen; G A Wilson; B N Fields; M R Neutra |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 68 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1994-12-12 Completed Date: 1994-12-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 8428-32 Citation Subset: IM |
Affiliation:
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GI Cell Biology Laboratory, Children's Hospital, Boston, Massachusetts 02115. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aprotinin / pharmacology* Capsid / physiology Endocytosis* Endopeptidases / metabolism* Epithelium / ultrastructure, virology Female Ileum Intestinal Mucosa / ultrastructure, virology* Mice Mice, Inbred BALB C Peyer's Patches / ultrastructure, virology Reoviridae / drug effects, physiology*, ultrastructure Viral Proteins / analysis, biosynthesis Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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P50 NS16998/NS/NINDS NIH HHS; R01 DK21505/DK/NIDDK NIH HHS; R37 HD17557/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Viral Proteins; 9087-70-1/Aprotinin; EC 3.4.-/Endopeptidases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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