Document Detail


Proteolytic processing of reovirus is required for adherence to intestinal M cells.
MedLine Citation:
PMID:  7525989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reovirus adheres specifically to apical membranes of mouse intestinal M cells and exploits M-cell transepithelial transport activity to enter Peyer's patch mucosa, where replication occurs. Proteolytic conversion of native reovirus to intermediate subviral particles (ISVPs) occurs in the intestine, but it is not known whether conversion is essential for interaction of virus with M cells. We tested the capacity of native virions, ISVPs, and cores (that lack outer capsid proteins) to bind to intestinal epithelial cells in vivo and found that only ISVPs adhered to M cells. Thus, intraluminal conversion of native reovirus to ISVPs is a prerequisite for M-cell adherence, and outer capsid proteins unique to ISVPs (either sigma 1 or products of mu 1) mediate interaction of virus with M-cell apical membranes.
Authors:
H M Amerongen; G A Wilson; B N Fields; M R Neutra
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  68     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1994-12-12     Completed Date:  1994-12-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  8428-32     Citation Subset:  IM    
Affiliation:
GI Cell Biology Laboratory, Children's Hospital, Boston, Massachusetts 02115.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aprotinin / pharmacology*
Capsid / physiology
Endocytosis*
Endopeptidases / metabolism*
Epithelium / ultrastructure,  virology
Female
Ileum
Intestinal Mucosa / ultrastructure,  virology*
Mice
Mice, Inbred BALB C
Peyer's Patches / ultrastructure,  virology
Reoviridae / drug effects,  physiology*,  ultrastructure
Viral Proteins / analysis,  biosynthesis
Virus Replication
Grant Support
ID/Acronym/Agency:
P50 NS16998/NS/NINDS NIH HHS; R01 DK21505/DK/NIDDK NIH HHS; R37 HD17557/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Viral Proteins; 9087-70-1/Aprotinin; EC 3.4.-/Endopeptidases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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