Document Detail


Proteolytic processing of the Aplysia egg-laying hormone prohormone.
MedLine Citation:
PMID:  9520477     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By using matrix-assisted laser desorption/ionization time-of-flight MS, individual peptidergic neurons from Aplysia are assayed. A semiquantitative method is developed for comparing single-cell profiles by using spectral normalization, and peptides are localized to specific cells by mass spectrometric cell mapping. In addition to all previously identified products of the egg-laying hormone (ELH) gene, other peptides are formed from proteolytic hydrolysis of Leu-Leu residues within ELH and acidic peptide (AP). AP exhibits further processing to yield AP1-20 and AP9-27. These peptides appear to be colocalized in vesicles with ELH, transported to specific neuronal targets, and released in a Ca2+-dependent manner. A differential peptide distribution is observed at a specific target cell, and a low-frequency variation of AP, [Thr21]AP, is detected in a single animal.
Authors:
R W Garden; S A Shippy; L Li; T P Moroz; J V Sweedler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  95     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-05-01     Completed Date:  1998-05-01     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3972-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Beckman Institute, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aplysia
Calcium / metabolism
Hydrolysis
Invertebrate Hormones / analysis*,  metabolism
Mass Spectrometry
Neurons / metabolism*
Grant Support
ID/Acronym/Agency:
1 R29NS31609/NS/NINDS NIH HHS; RR07141/RR/NCRR NIH HHS; RR10294/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Invertebrate Hormones; 0/egg-laying hormone, Mollusca; 7440-70-2/Calcium
Comments/Corrections

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