Document Detail

Proteolytic modification of swelling-activated Cl- current in LNCaP prostate cancer epithelial cells.
MedLine Citation:
PMID:  12392194     Owner:  NLM     Status:  MEDLINE    
The effects of intracellular application of trypsin on the Cl- current induced by hypotonic cell swelling (I(Cl,swell)) in human prostate cancer epithelial cells (LNCaP) was studied using the patch-clamp technique. In cells predialyzed with 1 mg/mL trypsin, I(Cl,swell)) developed and diminished in response to the application and withdrawal of hypotonic solution about three times faster than that in control cells. In trypsin-infused cells, I(Cl,swell)) also had about twofold higher current density and displayed considerably slowed voltage-dependent inactivation, which was quite pronounced in control cells at potentials above +60 mV. Trypsin-induced modification of I(Cl,swell)) could be prevented by coinfusion of 10 mg/mL soybean trypsin inhibitor, suggesting that proteolytic cleavage of essential intracellular structural domains of the I(Cl,swell))-carrying volume-regulated anion channel (VRAC) was responsible for this functional modification. The effect of trypsin was not dependent on the presence of intracellular ATP. We conclude that VRACs, similarly to voltage-gated Na+, K+, and Cl- channels, possess intracellular inactivation domain(s) subjected to proteolytic cleavage that may function in conformity with the classical "ball-and-chain" inactivation model.
Yulia V Vitko; Nelli H Pogorelaya; Natalia Prevarskaya; Roman Skryma; Yaroslav M Shuba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bioenergetics and biomembranes     Volume:  34     ISSN:  0145-479X     ISO Abbreviation:  J. Bioenerg. Biomembr.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-10-23     Completed Date:  2003-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7701859     Medline TA:  J Bioenerg Biomembr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  307-15     Citation Subset:  IM    
Bogomoletz Institute of Physiology, Kiev, Ukraine.
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MeSH Terms
Cell Size / physiology
Chloride Channels / metabolism*
Chlorine / metabolism*
Epithelium / pathology*
Hypotonic Solutions / pharmacology
Osmosis / physiology
Patch-Clamp Techniques
Prostatic Neoplasms / pathology*
Trypsin / metabolism*
Tumor Cells, Cultured
Reg. No./Substance:
0/Chloride Channels; 0/Hypotonic Solutions; 7782-50-5/Chlorine; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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