Document Detail

Proteolytic activity of lactobacilli in a model goats' milk curd system.
MedLine Citation:
PMID:  8987897     Owner:  NLM     Status:  MEDLINE    
Mesophyllic lactobacilli cultures propagated in MRS broth were inoculated in goats' milk curd slurries and incubated at 30 degrees C for 10 d. The micro-organisms tested were Lactobacillus casei subsp. casei IFPL 731 and IFPL 99, and Lactobacillus plantarum IFPL 3. Whole cells, cell-free extracts and cell lysates were evaluated for acceleration of proteolysis in the curd slurries. Conversion of water-soluble nitrogen to non-protein nitrogen and amino acid nitrogen, reverse phase-HPLC peak areas and ratio of hydrophobic to hydrophilic peptides, were all affected by the type of inoculum used as well as the strain under study. The results suggest that the accelerated-ripening model system developed, containing cell lysates, may be suitable as a good and rapid indicator of the contribution of the strains to proteolysis during cheese ripening.
L Parra; T Requena; V Casal; R Gómez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  23     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-02-03     Completed Date:  1997-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-8     Citation Subset:  B    
Instituto del Frio (CSIC), Ciudad Universitaria, Madrid, Spain.
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MeSH Terms
Amino Acids / metabolism
Cell Extracts / analysis
Cheese / microbiology
Chromatography, High Pressure Liquid
Culture Media / metabolism
Lactobacillus / enzymology*
Lactobacillus casei / enzymology*
Milk / metabolism
Nitrogen / metabolism
Peptide Hydrolases / analysis*
Peptides / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Cell Extracts; 0/Culture Media; 0/Peptides; 7727-37-9/Nitrogen; EC 3.4.-/Peptide Hydrolases

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