Document Detail

Proteolysis at pressure and HPLC peptide mapping of M4-lactate dehydrogenase homologs from marine fishes living at different depths.
MedLine Citation:
PMID:  1397507     Owner:  NLM     Status:  MEDLINE    
1. The effects at 10 degrees C of moderate hydrostatic pressure (136 atm) on trypsinolysis of muscle-type (M4) lactate dehydrogenase homologs (LDH, EC, L-lactate:NAD+ oxidoreductase) from shallow- and deep-occurring marine fishes were examined by mapping the partial digests by reverse phase HPLC. 2. Comparison of peptide maps of digests generated at 1 and 136 atm revealed that increased pressure did not expose new cleavage sites in homologs of any of the species; no new peptides were generated. 3. Increased pressure did alter the relative amounts of peptides produced. The net effect of increased pressure was to increase the amount of peptides generated in the shallow-occurring species. For deep-living species pressure did not alter the net amount of peptides produced compared to the 15 min atmospheric pressure samples, although the relative amounts of some of the peptides changed. Incubation at 136 atm for 30 min decreased the net amount of peptides produced. 4. It is suggested that the effects of pressure on trypsinolysis may result from slight conformational changes in the substrate proteins.
B J Davis; J F Siebenaller
Related Documents :
1397507 - Proteolysis at pressure and hplc peptide mapping of m4-lactate dehydrogenase homologs f...
18971557 - Rescue of pulmonary hypertension with an oral sulfonamide antibiotic sulfisoxazole by e...
2957477 - Relationship between plasma atrial natriuretic polypeptide concentration and hemodynami...
1299557 - Curative effects of highly active atrial natriuretic peptide on severe pregnancy-induce...
7947357 - Salt intake, blood pressure, and cardiovascular structure.
25273857 - The impact of age on the benefits and risks of aliskiren treatment: analyses of the 3a ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  24     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-11-17     Completed Date:  1992-11-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1135-9     Citation Subset:  IM; S    
Department of Zoology and Physiology, Louisiana State University, Baton Rouge 70803.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adaptation, Physiological / physiology*
Chromatography, High Pressure Liquid
Fishes / physiology*
Hydrostatic Pressure
L-Lactate Dehydrogenase / analysis*
Muscles / enzymology*
Peptide Mapping / methods*
Reg. No./Substance:
EC Dehydrogenase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Ribosomes from trichomonad protozoa have prokaryotic characteristics.
Next Document:  Purification and characterization of a glycosylated proline-rich protein from human parotid saliva.