Document Detail


Proteolysis at pressure and HPLC peptide mapping of M4-lactate dehydrogenase homologs from marine fishes living at different depths.
MedLine Citation:
PMID:  1397507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The effects at 10 degrees C of moderate hydrostatic pressure (136 atm) on trypsinolysis of muscle-type (M4) lactate dehydrogenase homologs (LDH, EC 1.1.1.27, L-lactate:NAD+ oxidoreductase) from shallow- and deep-occurring marine fishes were examined by mapping the partial digests by reverse phase HPLC. 2. Comparison of peptide maps of digests generated at 1 and 136 atm revealed that increased pressure did not expose new cleavage sites in homologs of any of the species; no new peptides were generated. 3. Increased pressure did alter the relative amounts of peptides produced. The net effect of increased pressure was to increase the amount of peptides generated in the shallow-occurring species. For deep-living species pressure did not alter the net amount of peptides produced compared to the 15 min atmospheric pressure samples, although the relative amounts of some of the peptides changed. Incubation at 136 atm for 30 min decreased the net amount of peptides produced. 4. It is suggested that the effects of pressure on trypsinolysis may result from slight conformational changes in the substrate proteins.
Authors:
B J Davis; J F Siebenaller
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  24     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-11-17     Completed Date:  1992-11-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1135-9     Citation Subset:  IM; S    
Affiliation:
Department of Zoology and Physiology, Louisiana State University, Baton Rouge 70803.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / physiology*
Animals
Chromatography, High Pressure Liquid
Fishes / physiology*
Hydrostatic Pressure
L-Lactate Dehydrogenase / analysis*
Muscles / enzymology*
Peptide Mapping / methods*
Seawater
Trypsin*
Chemical
Reg. No./Substance:
EC 1.1.1.27/L-Lactate Dehydrogenase; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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