Document Detail


Proteoglycan synthesis and osteophyte formation in 'metabolically' and 'mechanically' induced murine degenerative joint disease: an in-vivo autoradiographic study.
MedLine Citation:
PMID:  1320394     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the in-vivo proteoglycan synthesis in specific areas of murine knee joint articular cartilage after the induction of degenerative joint disease by means of 35S-sulphate autoradiography. Degenerative joint disease was induced either by direct interference with cartilage metabolism (papain and iodoacetate), or by the induction of joint instability (collagenase). Injection of iodoacetate and papain led to inhibition of proteoglycan synthesis mainly in the central parts of the patellae, patellaris femoris and the central part of the medial tibial plateau. Articular cartilage adjacent to the strongly inhibited areas frequently showed a significantly enhanced synthesis of proteoglycans. A strong inhibition of proteoglycan synthesis was observed in the central part of the medial plateau after collagenase injection while other cartilage sites and joint structures such as the capsule and ligaments were stimulated in their proteoglycan synthesis. This study shows that the localization of changes in cartilage metabolism in degenerative joint disease of the knee might be related to differences in the pathogenetic mechanism in different variants of this common joint disorder.
Authors:
P M van der Kraan; E L Vitters; H M van Beuningen; W B van den Berg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of experimental pathology     Volume:  73     ISSN:  0959-9673     ISO Abbreviation:  Int J Exp Pathol     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-08-12     Completed Date:  1992-08-12     Revised Date:  2012-05-07    
Medline Journal Info:
Nlm Unique ID:  9014042     Medline TA:  Int J Exp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  335-50     Citation Subset:  IM    
Affiliation:
Department of Rheumatology, University Hospital Nijmegen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage, Articular / metabolism,  pathology
Iodoacetates
Male
Mice
Mice, Inbred C57BL
Microbial Collagenase
Osteoarthritis / metabolism,  pathology*
Papain
Proteoglycans / biosynthesis
Time Factors
Chemical
Reg. No./Substance:
0/Iodoacetates; 0/Proteoglycans; EC 3.4.22.2/Papain; EC 3.4.24.3/Microbial Collagenase
Comments/Corrections

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