Document Detail

Proteoglycan synthesis and deposition in fetal rat bone.
MedLine Citation:
PMID:  6722109     Owner:  NLM     Status:  MEDLINE    
A pulse-labeling approach has been used to study proteoglycan metabolism in fetal rat bone. Pregnant rats were injected with [35S]sulfate and sacrificed 6, 24, or 48 h later. Fetal calvaria were dissected and extracted sequentially with 4 M guanidine hydrochloride and 4 M guanidine hydrochloride/0.5 M ( ethylenedinitrilo )tetraacetic acid (EDTA). With time after injection, the proportion of total incorporated radioactivity decreased in the guanidine pool (corresponding to nonmineralized bone and associated soft tissues) and increased in the guanidine/EDTA pool (mineralized bone). Chromatographic analysis of the proteoglycan species present in these pools after different labeling times indicated that three species of proteoglycan are synthesized in fetal rat calvaria. A large chondroitin sulfate (CS) proteoglycan and a smaller dermatan sulfate (DS) proteoglycan are located in the nonmineralized compartment. A CS proteoglycan similar in size to the DS proteoglycan is initially present in the nonmineralized bone but subsequently is located in the mineralized matrix. A fraction of the small CS proteoglycan is strongly associated with collagen.
G K Hunter; J N Heersche; J E Aubin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  23     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-06-22     Completed Date:  1984-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1572-6     Citation Subset:  IM    
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MeSH Terms
Bone Development*
Proteoglycans / biosynthesis*,  metabolism
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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