Document Detail

Proteinase-resistant factors in human erythrocyte membranes mediate CD4-dependent fusion with cells expressing human immunodeficiency virus type 1 envelope glycoproteins.
MedLine Citation:
PMID:  7815477     Owner:  NLM     Status:  MEDLINE    
Murine CD4+ cells are resistant to human immunodeficiency virus type 1 (HIV-1) entry and to fusion with cells expressing HIV-1 envelope glycoproteins (Env). The role of human-specific factors in Env/CD4-mediated fusion is shown by the ability of transient cell hybrids formed between CD4+ murine cells and human HeLa cells to fuse with Env+ cells. Fusion events were observed when other human cells, including erythrocytes, were substituted for HeLa cells in the hybrids. Experiments with erythrocyte ghosts showed that the factors allowing Env/CD4-mediated fusion are located in the plasma membrane. These factors were fully active after extensive digestion of erythrocytes with proteinase K or pronase. Nonprotein components of human plasma membranes, possibly glycolipids, could therefore be required for Env/CD4-mediated fusion and virus entry.
T Dragic; L Picard; M Alizon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  69     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-02-09     Completed Date:  1995-02-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1013-8     Citation Subset:  IM; X    
Institut National de la Santé et de la Recherche Médicale U332, Institut Cochin de Génétique Moléculaire, Paris, France.
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MeSH Terms
3T3 Cells
Antigens, CD4 / physiology*
Cell Line
Endopeptidase K
Erythrocyte Membrane / physiology*
Gene Products, env / physiology*
HIV-1 / physiology*
Leukemia, Erythroblastic, Acute / pathology
Membrane Fusion*
Pronase / pharmacology
Serine Endopeptidases / pharmacology
Reg. No./Substance:
0/Antigens, CD4; 0/Gene Products, env; EC 3.4.21.-/Serine Endopeptidases; EC K; EC 3.4.24.-/Pronase

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