Document Detail

Protein tyrosine phosphorylation in the cell cycle of BALB/c 3T3 fibroblasts.
MedLine Citation:
PMID:  3464948     Owner:  NLM     Status:  MEDLINE    
Cell cycle-dependent regulation of protein tyrosine phosphorylation in normal BALB/c 3T3 fibroblasts was examined by immunoblotting with a high-affinity antibody specific for phosphotyrosine. At least 15 different tyrosine-phosphorylated proteins are found in normal 3T3 cells. The level of tyrosine phosphorylation is higher in growing cells than in quiescent cells. However, a prominent tyrosine-phosphorylated protein of Mr 150,000 is present in quiescent cells, and its level is inversely proportional to the growth rate of these fibroblasts. Stimulation of quiescent cells with serum causes a major, yet transient, increase in tyrosine phosphorylation. The immediate tyrosine phosphorylation reactions in response to serum stimulation are independent of protein synthesis, but tyrosine phosphorylation reactions occurring later in the G1 phase of the cell cycle are inhibited by cycloheximide. Thus, tyrosine phosphorylation of proteins in normal 3T3 cells occurs predominantly at the G0 to G1 transition of the cell cycle. Maintenance of steady-state tyrosine phosphorylation is dependent on the presence of serum, but at least one tyrosine phosphorylation reaction occurs in the absence of cell growth.
A O Morla; J Y Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  83     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1986-12-05     Completed Date:  1986-12-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  8191-5     Citation Subset:  IM    
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MeSH Terms
Antibody Specificity
Cell Cycle
Cells, Cultured
Cycloheximide / pharmacology
Fibroblasts / metabolism
Mice, Inbred BALB C
Molecular Weight
Phosphoproteins / analysis*
Platelet-Derived Growth Factor / pharmacology
Tyrosine / immunology,  metabolism*
Reg. No./Substance:
0/Phosphoproteins; 0/Platelet-Derived Growth Factor; 55520-40-6/Tyrosine; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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