Document Detail


Protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus: sequence analysis, expression profile, and a possible biological role in host immunosuppression.
MedLine Citation:
PMID:  17363054     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A genome project has been launched and aims to sequence total genome of Cotesia plutellae bracovirus (CpBV). On this process, several putative open reading frames have been proposed, among which there was a large gene family coding for protein tyrosine phosphatases (PTPs). This study analyzed the deduced amino acid sequences of 14 CpBV-PTPs in terms of conserved domains with other known polydnaviral PTPs and determined their expression patterns in diamondback moth, Plutella xylostella, parasitized by C. plutellae. The analyzed CpBV-PTPs share the common 10 motifs with classical type of PTPs. However, there are variations among CpBV-PTPs in active site sequence and phosphorylation sites. Quantitative real-time polymerase chain reaction (PCR) indicated that most PTPs in the parasitized P. xylostella were expressed from the first day of parasitization and increased the expression levels during parasitization. All 14 PTPs were expressed in both immune-associated tissues of fat body and hemocytes in the parasitized host. During last instar, the PTP enzyme activity of the parasitized P. xylostella was significantly lower than that of the nonparasitized. The reduction of the PTP activity was observed in cytosolic fraction, but not in membrane fraction. The hemocytes of parasitized P. xylostella markedly lost their spreading ability in response to a cytokine (PSP1: plasmatocyte-spreading peptide 1). The functional link between the reduced PTP activity and the suppressed hemocytic behavior was evidenced by the inhibitory effect of sodium orthovanadate (a specific PTP inhibitor) on hemocyte-spreading behavior of nonparasitized P. xylostella. These results suggest that CpBV-PTPs are expressed in the parasitized P. xylostella and affect cellular PTP activity, which may be associated with host immunosuppression.
Authors:
Ahmed M A Ibrahim; Jae Young Choi; Yeon Ho Je; Yonggyun Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-13
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  31     ISSN:  0145-305X     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-30     Completed Date:  2007-10-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  978-90     Citation Subset:  IM    
Affiliation:
Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Gene Expression Regulation, Viral* / immunology
Immune Tolerance* / genetics
Immunity, Cellular
Molecular Sequence Data
Polydnaviridae / enzymology*,  genetics,  immunology
Protein Tyrosine Phosphatases / antagonists & inhibitors,  biosynthesis,  genetics*,  physiology
Viral Proteins / genetics
Wasps / immunology,  virology*
Chemical
Reg. No./Substance:
0/Viral Proteins; EC 3.1.3.48/Protein Tyrosine Phosphatases

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