| Protein typing of major outer membrane lipoproteins from Chinese pathogenic Leptospira spp. and characterization of their immunogenicity. | |
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MedLine Citation:
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PMID: 19796723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leptospirosis, caused by different Leptospira species, is one of the most widespread zoonotic infections worldwide. Here we expressed three major leptospiral lipoproteins and examined their immunogenicity. All the pathogenic Leptospira strains tested possess the lipL21, lipL32 and lipL41 genes, but the latter two can be further divided into different gene types (lipL32-1, lipL32-2, lipL41-1, lipL41-2). Microscopic agglutination test revealed that rLipLs antisera had extensive cross-immunoagglutination among the 178 leptospiral strains in which rLipL32-1 contributed the highest agglutination titer. The rLipLs-based ELISAs established in this study demonstrated that in the sera of 385 leptospirosis patients infected with different serovars of Leptospira interrogans, rLipL32-1 had the highest positive rates for IgG and IgM (89.4-98.7%), followed by the IgG/IgM positive rates of rLipL21 (87.0-96.1%) and rLipL32-2 (86.5-96.9%), while the two rLipL41s presented the lowest IgG/IgM positive rates (69.9-83.9%). The immunoprotective levels in guinea pigs of rLipL32-1 (58.3% and 66.7%) were the highest, compared to those of the other rLipLs (25.0-58.3%). Multiple different rLipLs would increase immunoprotective levels (from 58.3% and 66.7% to 83.3% and 91.7%). The data suggest that all the rLipLs are the genus-specific superficial antigens of pathogenic Leptospira species and should be considered in designing universal vaccines against leptospirosis. |
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Authors:
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Dongjiao Luo; Feng Xue; David M Ojcius; Jinfang Zhao; Yafei Mao; Liwei Li; Xuai Lin; Jie Yan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-29 |
Journal Detail:
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Title: Vaccine Volume: 28 ISSN: 1873-2518 ISO Abbreviation: Vaccine Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-01 Completed Date: 2010-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 243-55 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology and Parasitology, College of Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China. luoding9553@sohu.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Agglutination Tests Amino Acid Sequence Animals Antibodies, Bacterial / immunology Antigens, Bacterial / genetics, immunology* Bacterial Outer Membrane Proteins / genetics, immunology* Cross Reactions DNA, Bacterial / genetics Enzyme-Linked Immunosorbent Assay Guinea Pigs Humans Immunoglobulin G / immunology Immunoglobulin M / immunology Leptospira / genetics*, immunology Leptospirosis / immunology, prevention & control Lipoproteins / genetics, immunology* Molecular Sequence Data Phylogeny Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/DNA, Bacterial; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/LipL21 protein, Leptospira interrogans; 0/LipL32 protein, Leptospira; 0/LipL41 protein, Leptospira interrogans; 0/Lipoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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