Document Detail


Protein trafficking to the cell wall occurs through mechanisms distinguishable from default sorting in tobacco.
MedLine Citation:
PMID:  21223393     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The secretory pathway in plants involves sustained traffic to the cell wall, as matrix components, polysaccharides and proteins reach the cell wall through the endomembrane system. We studied the secretion pattern of cell-wall proteins in tobacco protoplasts and leaf epidermal cells using fluorescent forms of a pectin methylesterase inhibitor protein (PMEI1) and a polygalacturonase inhibitor protein (PGIP2). The two most representative protein fusions, secGFP-PMEI1 and PGIP2-GFP, reached the cell wall by passing through ER and Golgi stacks but using distinct mechanisms. secGFP-PMEI1 was linked to a glycosylphosphatidylinositol (GPI) anchor and stably accumulated in the cell wall, regulating the activity of the endogenous pectin methylesterases (PMEs) that are constitutively present in this compartment. A mannosamine-induced non-GPI-anchored form of PMEI1 as well as a form (PMEI1-GFP) that was unable to bind membranes failed to reach the cell wall, and accumulated in the Golgi stacks. In contrast, PGIP2-GFP moved as a soluble cargo protein along the secretory pathway, but was not stably retained in the cell wall, due to internalization to an endosomal compartment and eventually the vacuole. Stable localization of PGIP2 in the wall was observed only in the presence of a specific fungal endopolygalacturonase ligand in the cell wall. Both secGFP-PMEI1 and PGIP2-GFP sorting were distinguishable from that of a secreted GFP, suggesting that rigorous and more complex controls than the simple mechanism of bulk flow are the basis of cell-wall growth and differentiation.
Authors:
Monica De Caroli; Marcello S Lenucci; Gian-Pietro Di Sansebastiano; Giuseppe Dalessandro; Giulia De Lorenzo; Gabriella Piro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-01
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  65     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  295-308     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd.
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, 73100 Lecce, Italy.
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Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute

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