Document Detail


Protein-to-wet weight relationships in supragingival plaques from caries-prone tooth surfaces.
MedLine Citation:
PMID:  485148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ratio of protein to wet weight in unpooled samples of supragingival plaques from sound and carious tooth surfaces was studied. Protein was assayed by a procedure developed for the study, in order to achieve a sensitivity of 1 microgram with minimum effects upon quantitation from protein composition and nonprotein components. Ratios of protein to wet weight in plaque specimens from caries-free surfaces were almost equally distributed into two main categories of 9.4% and 6.5%. Corresponding values for specimens from carious surfaces were 9.1% and 5.0%. The occurrence of high and of low values among samples from each type of surface indicated that the plaques differed quantitatively in protein, water, or a nonprotein component, possibly extracellular polysaccharide. Although compositional differences between plaques from the two types of surfaces were indicated by the lower ratios of 6.5% from noncarious and 5.0% from carious surfaces, they were not indicated by the higher ratio values, which were similar. These results suggest either that protein-to-wet weight ratios are not related to caries, or that the ratio values are related to caries for some but not all types of plaques.
Authors:
M N Gilmour; G Turner; A K Krenzer; L B Zahn; J A Curzon
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  38     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-11-21     Completed Date:  1979-11-21     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  119-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis,  biosynthesis*
Child
Child, Preschool
Dental Caries / metabolism*
Dental Plaque / analysis*
Humans
Polysaccharides, Bacterial / analysis
Streptococcus sanguis / analysis,  metabolism*
Water / analysis
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Polysaccharides, Bacterial; 7732-18-5/Water
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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