Document Detail

Protein synthesis upon acute nutrient restriction relies on proteasome function.
MedLine Citation:
PMID:  16373576     Owner:  NLM     Status:  MEDLINE    
The mechanisms that protect mammalian cells against amino acid deprivation are only partially understood. We found that during an acute decrease in external amino acid supply, before up-regulation of the autophagosomal-lysosomal pathway, efficient translation was ensured by proteasomal protein degradation. Amino acids for the synthesis of new proteins were supplied by the degradation of preexisting proteins, whereas nascent and newly formed polypeptides remained largely protected from proteolysis. Proteasome inhibition during nutrient deprivation caused rapid amino acid depletion and marked impairment of translation. Thus, the proteasome plays a crucial role in cell survival after acute disruption of amino acid supply.
Ramunas M Vabulas; F Ulrich Hartl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  310     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-23     Completed Date:  2006-01-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1960-3     Citation Subset:  IM    
Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
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MeSH Terms
Amino Acids / metabolism*
Azetidinecarboxylic Acid / metabolism
Cell Line
Green Fluorescent Proteins / genetics
Hela Cells
Proteasome Endopeptidase Complex / antagonists & inhibitors,  physiology*
Protein Biosynthesis / physiology*
Protein Kinases / metabolism
Ubiquitin / genetics
Reg. No./Substance:
0/Amino Acids; 0/Ubiquitin; 147336-22-9/Green Fluorescent Proteins; 2517-04-6/Azetidinecarboxylic Acid; EC 2.7.-/Protein Kinases; EC Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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