Document Detail

Protein synthesis and morphogenesis are not tightly linked during embryogenesis in Fucus.
MedLine Citation:
PMID:  2472985     Owner:  NLM     Status:  MEDLINE    
Fertilized eggs of the brown alga Fucus have long been used as model organisms for investigating the early events in the establishment of polarity and subsequent embryogenesis since large numbers of zygotes can easily be obtained. We have analyzed protein synthesis in eggs and embryos during the first day of development using two-dimensional gels and found that synthesis of 12 of the 60 most prominent proteins changed either qualitatively or quantitatively. Actin and beta-tubulin were identified by immunoblotting; synthesis of these cytoskeletal proteins was initiated at different times during the first 12 hr of development. Unique, reproducible patterns of protein synthesis observed during development in the light permitted accurate staging of developing embryos. Inhibitors such as cytochalasin and sucrose, however, blocked morphogenesis without affecting protein synthesis, and, conversely, growth in the dark delayed protein synthesis but had very little effect on the timing of morphogenesis. The data are consistent with morphogenesis and protein synthesis being relatively independent during early embryogenesis. Actinomycin D added soon after fertilization had no effect on protein synthesis 1 day later, indicating that the proteins analyzed were encoded by maternal mRNA stored in the egg.
D L Kropf; R Hopkins; R S Quatrano
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  134     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-08-23     Completed Date:  1989-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  451-61     Citation Subset:  IM    
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.
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MeSH Terms
Algae / physiology*
Algae, Brown / cytology,  growth & development,  physiology*
Cell Division
Electrophoresis, Gel, Two-Dimensional
Protein Biosynthesis
Proteins / isolation & purification
RNA / biosynthesis
Reg. No./Substance:
0/Proteins; 63231-63-0/RNA

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