Document Detail


Protein synthesis inhibitor-mediated stability of adenomatous polyposis coli mRNA levels in HCT-116 colon cancer cells.
MedLine Citation:
PMID:  10339655     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the effect of a protein synthesis inhibitor, cycloheximide (CHX) on the adenomatous polyposis coli (APC) mRNA levels in HCT-116 colon cancer cell line. The HCT-116 cells were treated with different concentrations of CHX for 15 h. APC, p53 and beta-actin mRNA levels were determined by Northern blotting. Results showed that APC and beta-actin mRNA levels were significantly increased in a dose-dependent manner after CHX treatment. The p53 mRNA levels were moderately increased. The increase in APC mRNA levels after CHX treatment was due to increase in its stability instead of transcription. These results provide a model for CHX-induced APC mRNA stabilization and its implication in cell cycle arrest and cell survival studies.
Authors:
A S Jaiswal; S Narayan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  14     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-06-29     Completed Date:  1999-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  1045-8     Citation Subset:  IM    
Affiliation:
Sealy Center for Oncology and Hematology, and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555-1048, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenomatous Polyposis Coli Protein
Colonic Neoplasms / genetics,  metabolism*
Cycloheximide / pharmacology*
Cytoskeletal Proteins / antagonists & inhibitors,  biosynthesis*,  genetics
Dose-Response Relationship, Drug
Gene Expression Regulation, Neoplastic
Genes, APC / drug effects
Humans
Neoplasm Proteins / antagonists & inhibitors,  biosynthesis*,  genetics
Protein Synthesis Inhibitors / pharmacology*
RNA, Messenger / antagonists & inhibitors,  metabolism*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Adenomatous Polyposis Coli Protein; 0/Cytoskeletal Proteins; 0/Neoplasm Proteins; 0/Protein Synthesis Inhibitors; 0/RNA, Messenger; 66-81-9/Cycloheximide

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