Document Detail


Protein solubility modeling.
MedLine Citation:
PMID:  10397850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for the solution behavior describing deviation from ideal solution. Protein solubility is modeled as a function of salt concentration and temperature for a four-component system consisting of a protein, pseudo solvent (water and buffer), cation, and anion (salt). Two different systems, lysozyme with sodium chloride and concanavalin A with ammonium sulfate, are investigated. Comparison of the modeled and experimental protein solubility data results in an average root mean square deviation of 5.8%, demonstrating that the model closely follows the experimental behavior. Model calculations and model parameters are reviewed to examine the model and protein crystallization process.
Authors:
S M Agena; M L Pusey; I D Bogle
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  64     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-09-13     Completed Date:  1999-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  144-50     Citation Subset:  IM; S    
Copyright Information:
Copyright 1999 John Wiley & Sons, Inc.
Affiliation:
Biophysics ES76, National Aeronautics and Space Administration (NASA), Marshall Space Flight Center (MSFC), Huntsville, Alabama 35812, USA.
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MeSH Terms
Descriptor/Qualifier:
Ammonium Sulfate / chemistry
Concanavalin A / chemistry
Crystallization
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Kinetics
Models, Chemical*
Muramidase / chemistry
Proteins / chemistry*
Sodium Chloride / chemistry
Solubility
Temperature
Thermodynamics
Chemical
Reg. No./Substance:
0/Proteins; 11028-71-0/Concanavalin A; 7647-14-5/Sodium Chloride; 7783-20-2/Ammonium Sulfate; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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