Document Detail


Protein secretion pathways in Bacillus subtilis: implication for optimization of heterologous protein secretion.
MedLine Citation:
PMID:  16997527     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The absence of an outer membrane in Bacillus subtilis can simplify the protein secretion pathways and allow the organism to secrete high levels of extracellular proteins. Of the three known secretory routes, Sec-SRP pathway can direct the majority of secretory proteins into the growth medium. Alternatively, a small number of exoproteins with specific functions are secreted via Tat pathway or ABC transporters in B. subtilis. The discriminating function of precursor proteins among these pathways is largely attributed to the distinct structure of their cleavable signal peptides. Individual secretion machinery components with their special functions are involved in the total flow of proteins from the cytoplasm to the medium. Notably, multiple regulators with signal transduction functions can affect expression of secretion machinery as well as their post-transcriptional actions for protein secretion, resulting in the complicated networks in B. subtilis. Ultimately, according to the available knowledge of secretion machinery, several approaches aimed at optimizing protein secretion are discussed.
Authors:
Ling Lin Fu; Zi Rong Xu; Wei Fen Li; Jiang Bing Shuai; Ping Lu; Chun Xia Hu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2006-08-15
Journal Detail:
Title:  Biotechnology advances     Volume:  25     ISSN:  0734-9750     ISO Abbreviation:  Biotechnol. Adv.     Publication Date:    2007 Jan-Feb
Date Detail:
Created Date:  2007-01-08     Completed Date:  2007-06-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8403708     Medline TA:  Biotechnol Adv     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-12     Citation Subset:  IM    
Affiliation:
Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University (Hua Jia Chi Campus), Hangzhou 310029, China.
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / metabolism
Bacillus subtilis / metabolism*
Bacterial Proteins / chemistry,  secretion*
Humans
Metabolic Networks and Pathways
Protein Transport
Signal Recognition Particle
Chemical
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Bacterial Proteins; 0/Signal Recognition Particle

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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