| Protein secretion by the mouse blastocyst: differences in the polypeptide composition secreted into the blastocoel and medium. | |
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MedLine Citation:
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PMID: 1786298 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To initiate studies on the protein microenvironment of the mouse blastocoel, we examined the electrophoretic profile of newly synthesized proteins secreted into the blastocoel, as well as those secreted into the medium. Although most polypeptides reveal no relative enrichment, some proteins (e.g., proteins of Mr = 155,000 and 33,000) are enriched in the blastocoel relative to those secreted apically into the medium. In addition, some proteins (e.g., proteins of Mr = 102,000 and 40,000) are enriched in the medium relative to the blastocoel. The relative amount of newly synthesized protein secreted into the blastocoel is about 2.5% of total protein synthesis. In addition, the trophectoderm and inner cell mass contribute to these proteins. Results of these studies suggest a potential function for these blastocoel-enriched proteins in inner cell mass development. |
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Authors:
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A Dardik; R M Schultz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 45 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1991 Aug |
Date Detail:
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Created Date: 1992-03-20 Completed Date: 1992-03-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 328-33 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Pennsylvania, Philadelphia 19104-6018. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / drug effects, secretion* Body Fluids / chemistry Culture Media / analysis Cytochalasin B / pharmacology Electrophoresis, Polyacrylamide Gel Female Mice Molecular Weight Peptide Mapping Peptides / secretion Proteins / secretion* |
| Grant Support | |
ID/Acronym/Agency:
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HD 22681/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Peptides; 0/Proteins; 14930-96-2/Cytochalasin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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