Document Detail


Protein responses in blue mussels (Mytilus edulis) exposed to organic pollutants: a combined CYP-antibody/proteomic approach.
MedLine Citation:
PMID:  16603256     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyclonal antibodies were raised against highly conserved, trans-metazoan sequences of cytochrome P450 (CYP) families 2 and 4 and used to investigate responses in the common blue mussel (Mytilus edulis) exposed to various organic contaminants. The results were evaluated by means of cross-reacting proteins on Western blots of both one- and two-dimensional electrophoresis gels, and by scanning spectroscopy measurements of total CYP content. Furthermore, a proteomic approach was applied aimed at elucidating exposure-related protein changes in a more general term. Identities of isolated proteins were searched by means of peptide mass fingerprints obtained from MALDI-TOF MS analyses. The results demonstrated that both antibodies rendered several cross-reactive bands when probed on Western blots. The most obvious cross-reaction of the CYP2 antibody was with a strongly expressed protein of size approximately 57kDa, pI 4.5-4.6, whereas the CYP4 antibody cross-reacted with a protein of size approximately 55kDa, pI 5.6. However, expression of cross-reacting proteins did not change as a result of the exposures, and resulted only in small and insignificant fluctuations in total CYP content. As a contrast, silver-stained 2DE gels showed that several microsomal proteins were affected in individuals exposed to diallylphthalate as well as crude oil, with and without a spike of alkylphenols and PAHs. Mass spectrometry based analyses of excised, trypsin-digested spots did so far not decipher the identities of the proteins affected by the exposures, nor of those cross-reacting with CYP2 and CYP4 antibodies. This study has underlined the power of the proteomic approach in environmental toxicology, although protein identification was not successful. The missing identities of the proteins cross-reacting with the CYP2- and CYP4-antibodies does not enable a clear conclusion as to whether or not these peptides actually represent CYP iso-enzymes.
Authors:
Henrik Jonsson; Doris Schiedek; Bjørn Einar Grøsvik; Anders Goksøyr
Related Documents :
6531966 - Biochemical and immunological analyses of the cell surface of bordetella bronchiseptica...
22844406 - Adp ribosylation by parp-1 suppresses hoxb7 transcriptional activity.
21524186 - A new monitoring protocol for clozapine-induced myocarditis based on an analysis of 75 ...
11478446 - Studies on chicken acquired resistance to argas (persicargas) persicus latereille (acar...
3519546 - An immunofluorescent method for a specific demonstration of granulocytes and some of th...
1297326 - Extrusion of food proteins.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-17
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  78 Suppl 1     ISSN:  0166-445X     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-12     Completed Date:  2007-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  S49-56     Citation Subset:  IM    
Affiliation:
Baltic Sea Research Institute, Rostock, Germany. jonsson@unipmn.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies / immunology
Blotting, Western / methods
Cross Reactions
Cytochrome P-450 Enzyme System / chemistry,  immunology*,  metabolism
Electrophoresis, Gel, Two-Dimensional
Environmental Exposure
Female
Flounder
Gene Expression Profiling / methods
Gene Expression Regulation / drug effects*
Liver / drug effects
Male
Microsomes / drug effects,  immunology,  metabolism
Mytilus edulis / drug effects*
Oncorhynchus mykiss
Organic Chemicals / toxicity*
Petroleum
Proteomics / methods
Water Pollutants, Chemical / toxicity*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Organic Chemicals; 0/Petroleum; 0/Water Pollutants, Chemical; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Investigation of micronuclei and other nuclear abnormalities in peripheral blood and kidney of marin...
Next Document:  Isolation and characterization of Schistosoma mansoni constitutive androstane receptor.