Document Detail

Protein quantification of UDP-glucuronosyltransferases 1A1 and 2B7 in human liver microsomes by LC-MS/MS and correlation with glucuronidation activities.
MedLine Citation:
PMID:  22435749     Owner:  NLM     Status:  MEDLINE    
The aims of this study were to quantify absolute protein levels of uridine 5'-diphosphate-glucuronosyltransferases (UGTs) 1A1 and 2B7 in human liver microsomes (HLMs) and to investigate their correlation with marker activities. A quantification method for UGT1A1 and UGT2B7 in HLMs was developed. Unique tryptic peptides of UGT1A1 and UGT2B7 in tryptically digested HLMs were simultaneously quantified by liquid chromatography (LC) equipped with tandem mass spectrometry (MS) using corresponding stable isotope-labelled peptides as internal standards. Bovine serum albumin was used as a blank matrix for calibration curve samples. Our procedure had good digestion efficiency, sensitivity, calibration curve linearity, and reproducibility of digestion to quantification. In 16 individual HLMs, the protein levels of UGT1A1 and UGT2B7 ranged from 6.50 to 44.6 pmol/mg and 4.45 to 18.2 pmol/mg, respectively. Estradiol 3β-glucuronidation correlated strongly with the UGT1A1 level, indicating its high reliability as a reaction marker. Both morphine 3-O- and 6-O-glucuronidation significantly correlated with UGT2B7 level. However, the intercept of the linear regression clearly indicates that morphine glucuronidation was mediated by other UGT isoforms in addition to UGT2B7.
Yuichiro Sato; Masanori Nagata; Akio Kawamura; Aiji Miyashita; Takashi Usui
Related Documents :
21386209 - Lc-esi-ms/ms method for oxidative stress multimarker screening in the exhaled breath co...
21723909 - Chemical characterization of iraqi propolis samples and assessing their antioxidant pot...
21796869 - In vitro antimicrobial and antioxidant activity of acetone and methanol extracts from t...
21612369 - Secondary metabolites of centaurea calolepis and evaluation of cnicin for anti-inflamma...
8822839 - Identification of c31 and c32 sterols in pneumocystis carinii hominis-infected human lu...
581309 - Evaluation of some alternative mechanisms for interface-related stress relaxation in lung.
17723529 - Analysis of phenolic acids as chloroformate derivatives using solid phase microextracti...
18244069 - Biomechanical quantification for assessment and diagnosis of dysphagia.
263319 - Extraction of human chorionic thyrotropin (hct) from term placentas: failure to recover...
Publication Detail:
Type:  Journal Article     Date:  2012-03-21
Journal Detail:
Title:  Xenobiotica; the fate of foreign compounds in biological systems     Volume:  42     ISSN:  1366-5928     ISO Abbreviation:  Xenobiotica     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-08     Completed Date:  2012-12-10     Revised Date:  2014-03-28    
Medline Journal Info:
Nlm Unique ID:  1306665     Medline TA:  Xenobiotica     Country:  England    
Other Details:
Languages:  eng     Pagination:  823-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Markers / analysis
Blotting, Western
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Estradiol / analogs & derivatives,  metabolism
Glucuronosyltransferase / analysis*
Isotope Labeling
Microsomes, Liver / enzymology*
Tandem Mass Spectrometry
Reg. No./Substance:
0/Biological Markers; 15270-30-1/estradiol-3-glucuronide; 4TI98Z838E/Estradiol; EC 2.4.1.-/UGT1A1 enzyme; EC 2.4.1.-/UGT2B7 protein, human; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Systematic review of comparative effectiveness data for oncology orphan drugs.
Next Document:  Adverse associations of increases in television viewing time with 5-year changes in glucose homoeost...