| Protein-protein ratchets: stochastic simulation and application to processive enzymes. | |
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MedLine Citation:
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PMID: 11509349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interaction between a protein and a series of binding sites on a cytoskeletal substrate can create a resistance, or "protein friction," as the protein is moved along the substrate. If attachment and detachment rates are specified asymmetrically, this resistance can depend on the direction of movement, and the binding interaction acts as a ratchet. Stochastic computer simulations have been used to examine this type of protein-protein interaction. The performance of a protein-protein ratchet in the piconewton and nanometer range is significantly limited by thermal fluctuations, which in experimental measurements with single molecules are evident as Brownian motion. Simulations with a two-component model combining a conventional motor enzyme model with a protein-protein ratchet confirm previous suggestions that the processive movement of a single motor enzyme molecule against a load, as seen in experiments with inner arm dynein molecules, might be made possible by an accessory protein interaction that prevents backward slippage. When this accessory protein interaction is defined so that it acts as a ratchet, backward slippage can be prevented with minimal interference with forward progression. |
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Authors:
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C J Brokaw |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biophysical journal Volume: 81 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-08-17 Completed Date: 2001-10-18 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 1333-44 Citation Subset: IM |
Affiliation:
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Division of Biology, California Institute of Technology, Pasadena, California 91125, USA. brokawc@its.caltech.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Biomechanics Computer Simulation* Cytoskeleton / metabolism* Dyneins / chemistry, metabolism Enzymes / chemistry*, metabolism* Friction Models, Biological* Molecular Motor Proteins / chemistry, metabolism Monte Carlo Method Movement Protein Binding Stochastic Processes |
| Chemical | |
Reg. No./Substance:
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0/Enzymes; 0/Molecular Motor Proteins; EC 3.6.4.2/Dyneins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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