Document Detail

Protein-protein ratchets: stochastic simulation and application to processive enzymes.
MedLine Citation:
PMID:  11509349     Owner:  NLM     Status:  MEDLINE    
Interaction between a protein and a series of binding sites on a cytoskeletal substrate can create a resistance, or "protein friction," as the protein is moved along the substrate. If attachment and detachment rates are specified asymmetrically, this resistance can depend on the direction of movement, and the binding interaction acts as a ratchet. Stochastic computer simulations have been used to examine this type of protein-protein interaction. The performance of a protein-protein ratchet in the piconewton and nanometer range is significantly limited by thermal fluctuations, which in experimental measurements with single molecules are evident as Brownian motion. Simulations with a two-component model combining a conventional motor enzyme model with a protein-protein ratchet confirm previous suggestions that the processive movement of a single motor enzyme molecule against a load, as seen in experiments with inner arm dynein molecules, might be made possible by an accessory protein interaction that prevents backward slippage. When this accessory protein interaction is defined so that it acts as a ratchet, backward slippage can be prevented with minimal interference with forward progression.
C J Brokaw
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biophysical journal     Volume:  81     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-08-17     Completed Date:  2001-10-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1333-44     Citation Subset:  IM    
Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.
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MeSH Terms
Binding Sites
Computer Simulation*
Cytoskeleton / metabolism*
Dyneins / chemistry,  metabolism
Enzymes / chemistry*,  metabolism*
Models, Biological*
Molecular Motor Proteins / chemistry,  metabolism
Monte Carlo Method
Protein Binding
Stochastic Processes
Reg. No./Substance:
0/Enzymes; 0/Molecular Motor Proteins; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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