Document Detail


Protein profiling underscores immunological functions of uterine cervical mucus plug in human pregnancy.
MedLine Citation:
PMID:  21362502     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cervical mucus plug (CMP) differs from the cervical secretions of non-pregnant women, and is the ultimate sealant of the uterine cavity during pregnancy. Although several studies have analyzed biochemical properties of large glycoproteins in the CMP, comprehensive information about its protein composition is yet unavailable. We hypothesized that protein profiling of the CMP could provide key clues to its physiological functions in pregnancy. For this purpose, five CMPs obtained from women in labor at term were analyzed by LC-MS/MS. Out of 291 total proteins identified, 137 were detected in two or more samples, which included S100A8, S100A9, and complement proteins (C3, C4a, C4b, C6, and C8g). Several proteins, which have not been described in the cervical mucus of non-pregnant women or in cervicovaginal fluids, such as CD81 antigen and pregnancy zone protein, were also identified. Gene ontology analysis of identified proteins showed significant enrichment of 28 biological processes such as 'activation of plasma proteins involved in acute inflammatory response' and 'positive regulation of cholesterol esterification'. We report the proteome of CMPs from pregnant women at term for the first time, and the overall findings strongly suggest an important role for the CMP in the maintenance of pregnancy and parturition.
Authors:
Deug-Chan Lee; Sonia S Hassan; Roberto Romero; Adi L Tarca; Gaurav Bhatti; Maria Teresa Gervasi; Joseph A Caruso; Paul M Stemmer; Chong Jai Kim; Lea Kirstine Hansen; Naja Becher; Niels Uldbjerg
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2011-03-23
Journal Detail:
Title:  Journal of proteomics     Volume:  74     ISSN:  1876-7737     ISO Abbreviation:  J Proteomics     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-08-15     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101475056     Medline TA:  J Proteomics     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  817-28     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, Maryland, and Detroit, MI, USA.
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MeSH Terms
Descriptor/Qualifier:
Cervix Mucus / chemistry*,  immunology*
Female
Humans
Labor, Obstetric / metabolism
Mass Spectrometry
Parturition / metabolism
Pregnancy / physiology*
Protein Array Analysis
Proteomics / methods
Grant Support
ID/Acronym/Agency:
P30-ES006639/ES/NIEHS NIH HHS; ZIA HD002400-19/HD/NICHD NIH HHS
Comments/Corrections

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