Document Detail


Protein profile of human lung squamous carcinoma cell line NCI-H226.
MedLine Citation:
PMID:  17458138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. METHOD: Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. RESULTS: The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95 +/- 0.53 mm in the isoelectric focusing direction, and 1.73 +/- 0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis. CONCLUSION: The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer.
Authors:
Hao Zhang; Na Li; Yue Chen; Ling-Yun Huang; Yi-Ching Wang; Gang Fang; Da-Cheng He; Xue-Yuan Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical and environmental sciences : BES     Volume:  20     ISSN:  0895-3988     ISO Abbreviation:  Biomed. Environ. Sci.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-04-26     Completed Date:  2007-06-29     Revised Date:  2007-07-16    
Medline Journal Info:
Nlm Unique ID:  8909524     Medline TA:  Biomed Environ Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24-32     Citation Subset:  IM    
Affiliation:
Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Universities' Confederated Institute of Proteomics, Beijing Normal University, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / chemistry*
Cell Line, Tumor*
Databases, Factual
Electrophoresis, Gel, Two-Dimensional
Humans
Lung Neoplasms / chemistry*
Neoplasm Proteins / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry
Tumor Markers, Biological / analysis*
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Tumor Markers, Biological
Comments/Corrections
Erratum In:
Biomed Environ Sci. 2007 Apr;20(2):163

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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