| Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves. | |
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MedLine Citation:
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PMID: 20358221 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the roles of protein phosphatase type 2A catalytic subunits (PP2Ac) in the leaf responses to conditions that affect tuberization. The responses were monitored by analyzing the expression of the "tuber-specific" genes Patatin and Pin2, which are induced in tubers and leaves during tuber induction. Experiments using PP2A inhibitors, together with PP2Ac expression profiles under conditions that affect tuberization indicate that high sucrose/nitrogen ratio, which promotes tuber formation, increases the transcript levels of Patatin and Pin2, by increasing the activity of PP2As without affecting PP2Ac mRNA or protein levels. Gibberellic acid (GA), a negative regulator of tuberization, down-regulates the transcription of catalytic subunits of PP2As from the subfamily I and decreases their enzyme levels. In addition, GA inhibits the expression of Patatin and Pin2 possibly by a PP2A-independent mechanism. PP2Ac down-regulation by GA may inhibit tuberization signaling downstream of the inductive effects of high sucrose/nitrogen ratio. These results are consistent with the hypothesis that PP2As of the subfamily I may positively modulate the signaling pathways that lead to the transcriptional activation of "tuber-specific" genes in leaves, and act as molecular switches regulated by both positive and negative modulators of tuberization. |
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Authors:
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Silvia Marina País; María Noelia Muñiz García; María Teresa Téllez-Iñón; Daniela Andrea Capiati |
Publication Detail:
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Type: Journal Article Date: 2010-04-01 |
Journal Detail:
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Title: Planta Volume: 232 ISSN: 1432-2048 ISO Abbreviation: Planta Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-18 Completed Date: 2010-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 37-49 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves / enzymology, metabolism* Protein Phosphatase 2 / chemistry, metabolism* Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction* Solanum tuberosum / enzymology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; EC 3.1.3.16/Protein Phosphatase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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