Document Detail

Protein patterns, osmolytes, and aldose reductase of L-929 cells exposed to hyperosmotic media.
MedLine Citation:
PMID:  8647909     Owner:  NLM     Status:  MEDLINE    
L-929 cells acclimated to media made hyperosmotic (600 mosmol/kgH2O) by addition of NaCl, sorbitol, or mannitol show, on SDS-polyacrylamide gels, a markedly enhanced protein band at 40 kDa, most likely corresponding to the enzyme aldose reductase. The effect was not observed in cells acclimated to a medium rendered hyperosmotic by addition of proline. The major organic osmolyte accumulated is sorbitol in cells acclimated to high-sorbitol or high-NaCl medium, proline in cells acclimated to high-proline medium. Cells acclimated to any of these hyperosmotic media display unaltered Na+ levels and similarly increased K+ levels and decreased Cl-levels. These results are interpreted in terms of the mechanisms involved in aldose reductase induction and in regulation of the enzyme activity in long-term acclimation to hyperosmotic media.
C Libioulle; G Llabres; R Gilles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  168     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-07-25     Completed Date:  1996-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  147-54     Citation Subset:  IM    
Laboratory of Animal Physiology, University of Liège, Belgium.
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MeSH Terms
Adaptation, Physiological
Aldehyde Reductase / metabolism*
L Cells (Cell Line)
Molecular Weight
Proteins / chemistry,  metabolism
Sorbitol / metabolism
Time Factors
Water-Electrolyte Balance*
Reg. No./Substance:
0/Proteins; 50-70-4/Sorbitol; EC Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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