| Protein oxidation in plant mitochondria detected as oxidized tryptophan. | |
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MedLine Citation:
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PMID: 16443157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The formation of N-formylkynurenine by dioxygenation of tryptophan was detected in peptides from rice leaf and potato tuber mitochondria. Proteins in matrix and membrane fractions were separated by two-dimensional gel electrophoresis and identified using a Q-TOF mass spectrometer. N-Formylkynurenine was detected in 29 peptides representing 17 different proteins. With one exception, the oxidation-sensitive aconitase, all of these proteins were either redox active themselves or subunits in redox-active enzyme complexes. The same site was modified in (i) several adjacent spots containing the P protein of the glycine decarboxylase complex, (ii) two different isoforms of the mitochondrial processing peptidase in complex III, and (iii) the same tryptophan residues in Mn-superoxide dismutase in both rice and potato mitochondria. This indicates that Trp oxidation is a selective process. |
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Authors:
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Ian M Møller; Brian K Kristensen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-11-09 |
Journal Detail:
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Title: Free radical biology & medicine Volume: 40 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-30 Completed Date: 2006-05-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 430-5 Citation Subset: IM |
Affiliation:
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Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. imm@kvl.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aconitate Hydratase
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metabolism Amino Acid Sequence Electrophoresis, Gel, Two-Dimensional Kynurenine / analogs & derivatives, metabolism Mitochondria / metabolism* Mitochondrial Proteins / chemistry* Molecular Sequence Data Oxidation-Reduction Plant Leaves / chemistry Plant Proteins / chemistry* Sequence Homology, Amino Acid Solanum tuberosum / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Superoxide Dismutase Tryptophan / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Mitochondrial Proteins; 0/Plant Proteins; 1022-31-7/N'-formylkynurenine; 343-65-7/Kynurenine; 73-22-3/Tryptophan; EC 1.15.1.1/Superoxide Dismutase; EC 4.2.1.3/Aconitate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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