| Protein and messenger RNA levels of plasminogen activators and inhibitors analyzed in 22 human tumor cell lines. | |
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MedLine Citation:
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PMID: 2105839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase (u-PA) and tissue-type (t-PA) and their inhibitors PAI-1 and PAI-2 was studied. These four components may determine the net plasminogen activator activity, which is often associated with tumor development and metastatic processes. The amount of specific mRNA and protein produced by the cells was measured for all four components. The frequent finding of t-PA (alone or in combination with u-PA) suggests that t-PA can also be a tumor-associated plasminogen activator. In 11 of the 22 cells PAI-1 mRNA and in 6 of the 22 cells PAI-2 mRNA was found, pointing to a possible role of plasminogen activator inhibitors in the tumor-related plasminogen activator activity. This study demonstrates that there are at least two important regulatory steps in the regulation of production of plasminogen activators and their inhibitors: (a) the regulation at the mRNA level, since a high protein amount is always correlated with a high mRNA amount found in the tumor cells; (b) there must be a significant regulatory step at the (post)translational level as can be concluded from differences in mRNA usage. |
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Authors:
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P H Quax; R T van Leeuwen; H W Verspaget; J H Verheijen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer research Volume: 50 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-03-21 Completed Date: 1990-03-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1488-94 Citation Subset: IM |
Affiliation:
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Gaubius Institute TNO, Leiden, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Humans Plasminogen Activators / analysis*, genetics, metabolism Plasminogen Inactivators / analysis* RNA, Messenger / analysis* Tissue Plasminogen Activator / analysis*, genetics, metabolism Transcription, Genetic* Tumor Cells, Cultured / analysis, metabolism Urokinase-Type Plasminogen Activator / analysis*, genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Plasminogen Inactivators; 0/RNA, Messenger; EC 3.4.21.-/Plasminogen Activators; EC 3.4.21.68/Tissue Plasminogen Activator; EC 3.4.21.73/Urokinase-Type Plasminogen Activator |
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