Document Detail


Protein labeling with the labeling precursor [(18)F]SiFA-SH for positron emission tomography.
MedLine Citation:
PMID:  23037311     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteins previously derivatized with the cross-coupling reagent sulfo-SMCC (4-(N-maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxy-succinimide ester sodium salt) can be easily labeled in high radiochemical yields with the silicon-fluoride acceptor (SiFA) reagent [(18)F]SiFA-SH, obtained via isotopic exchange, by thiol-maleimide coupling chemistry (n = 10). The specific activity of SiFA-SH obtained in a one-step labeling reaction was > 18.5 GBq μmol(-1) (> 500 Ci mmol(-1)). The number of SiFA building blocks per protein molecule is defined by the previously introduced number of maleimide groups, which can be determined by a simple and convenient Ellman's assay. Not more than two maleimide groups are introduced using sulfo-SMCC, thereby keeping the modification of the protein low and preserving its biological activity.
Authors:
Björn Wängler; Alexey P Kostikov; Sabrina Niedermoser; Joshua Chin; Katy Orchowski; Esther Schirrmacher; Liuba Iovkova-Berends; Klaus Jurkschat; Carmen Wängler; Ralf Schirrmacher
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-04
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-05     Completed Date:  2013-03-19     Revised Date:  2013-03-21    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1964-9     Citation Subset:  IM    
Affiliation:
Molecular Imaging and Radiochemistry, Medical Faculty Mannheim, Department of Clinical Radiology and Nuclear Medicine, Heidelberg University, Mannheim, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Fluorine Radioisotopes / chemistry*
Isotope Labeling / methods*
Organosilicon Compounds / chemical synthesis*
Positron-Emission Tomography / methods*
Proteins / chemistry*
Chemical
Reg. No./Substance:
0/4-(di-tert-butyl(18F)fluorosilyl)benzenethiol; 0/Fluorine Radioisotopes; 0/Organosilicon Compounds; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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