Document Detail

Protein kinase VRK-1 regulates cell invasion and EGL-17/FGF signaling in Caenorhabditis elegans.
MedLine Citation:
PMID:  19679119     Owner:  NLM     Status:  MEDLINE    
The vaccinia-related kinases (VRKs) are highly conserved throughout the animal kingdom and phosphorylate several chromatin proteins and transcription factors. In early Caenorhabditis elegans embryos, VRK-1 is required for proper nuclear envelope formation. In this work, we present the first investigation of the developmental role of VRKs by means of a novel C. elegans vrk-1 mutant allele. We found that VRK-1 is essential in hermaphrodites for formation of the vulva, uterus, and utse and for development and maintenance of the somatic gonad and thus the germ line. VRK-1 regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage. VRK-1 is also required for proper specification and proliferation of uterine cells and sex myoblasts. Expression of the fibroblast growth factor-like protein EGL-17 and its receptor EGL-15 is reduced in vrk-1 mutants, suggesting that VRK-1 might act at least partially through activation of FGF signaling. Expression of a translational VRK-1Colon, two colonsGFP fusion protein in the ventral nerve cord and vulva precursor cells restores vulva and uterus formation, suggesting both cell autonomous and non-autonomous roles of VRK-1.
Elke P F Klerkx; Pilar Alarcón; Katherine Waters; Valerie Reinke; Paul W Sternberg; Peter Askjaer
Related Documents :
9545199 - Wing development and specification of dorsal cell fates in the absence of apterous in d...
8026629 - The function of argos in regulating cell fate decisions during drosophila eye and wing ...
18213359 - Stage-specific differences in the requirements for germline stem cell maintenance in th...
9790529 - Partner of numb colocalizes with numb during mitosis and directs numb asymmetric locali...
15936339 - A novel signal induces a segmentation fissure by acting in a ventral-to-dorsal directio...
16751349 - The bam1/bam2 receptor-like kinases are important regulators of arabidopsis early anthe...
15664929 - Solubility and bioactivity of the pseudomonas quinolone signal are increased by a pseud...
10543799 - Survival, physiology, and lysis of lactococcus lactis in the digestive tract.
10697559 - Confocal spectral imaging analysis of intracellular interactions of mitoxantrone at dif...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-08-11
Journal Detail:
Title:  Developmental biology     Volume:  335     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-19     Completed Date:  2009-12-14     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12-21     Citation Subset:  IM    
Centro Andaluz de Biología del Desarrollo (CABD), Consejo Superior de Investigaciones Científicas, Universidad Pablo de Olavide, Seville 41013, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Caenorhabditis elegans* / anatomy & histology,  embryology,  physiology
Caenorhabditis elegans Proteins / genetics,  metabolism*
Fibroblast Growth Factors / genetics,  metabolism*
Gene Expression Regulation, Developmental
Intercellular Signaling Peptides and Proteins / genetics,  metabolism*
Protein-Serine-Threonine Kinases / genetics,  metabolism*
RNA Interference
Receptors, Fibroblast Growth Factor / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Signal Transduction / physiology*
Grant Support
T32 GM007499/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/EGL-15 protein, C elegans; 0/Egl-17 protein, C elegans; 0/Intercellular Signaling Peptides and Proteins; 0/Receptors, Fibroblast Growth Factor; 0/Recombinant Fusion Proteins; 62031-54-3/Fibroblast Growth Factors; EC Kinases; EC protein, C elegans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The cis-regulatory system of the tbrain gene: Alternative use of multiple modules to promote skeleto...
Next Document:  Arabidopsis actin-related protein ARP5 in multicellular development and DNA repair.