Document Detail


Protein kinase VRK-1 regulates cell invasion and EGL-17/FGF signaling in Caenorhabditis elegans.
MedLine Citation:
PMID:  19679119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The vaccinia-related kinases (VRKs) are highly conserved throughout the animal kingdom and phosphorylate several chromatin proteins and transcription factors. In early Caenorhabditis elegans embryos, VRK-1 is required for proper nuclear envelope formation. In this work, we present the first investigation of the developmental role of VRKs by means of a novel C. elegans vrk-1 mutant allele. We found that VRK-1 is essential in hermaphrodites for formation of the vulva, uterus, and utse and for development and maintenance of the somatic gonad and thus the germ line. VRK-1 regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage. VRK-1 is also required for proper specification and proliferation of uterine cells and sex myoblasts. Expression of the fibroblast growth factor-like protein EGL-17 and its receptor EGL-15 is reduced in vrk-1 mutants, suggesting that VRK-1 might act at least partially through activation of FGF signaling. Expression of a translational VRK-1Colon, two colonsGFP fusion protein in the ventral nerve cord and vulva precursor cells restores vulva and uterus formation, suggesting both cell autonomous and non-autonomous roles of VRK-1.
Authors:
Elke P F Klerkx; Pilar Alarcón; Katherine Waters; Valerie Reinke; Paul W Sternberg; Peter Askjaer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-08-11
Journal Detail:
Title:  Developmental biology     Volume:  335     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-19     Completed Date:  2009-12-14     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12-21     Citation Subset:  IM    
Affiliation:
Centro Andaluz de Biología del Desarrollo (CABD), Consejo Superior de Investigaciones Científicas, Universidad Pablo de Olavide, Seville 41013, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans* / anatomy & histology,  embryology,  physiology
Caenorhabditis elegans Proteins / genetics,  metabolism*
Fibroblast Growth Factors / genetics,  metabolism*
Gene Expression Regulation, Developmental
Intercellular Signaling Peptides and Proteins / genetics,  metabolism*
Protein-Serine-Threonine Kinases / genetics,  metabolism*
RNA Interference
Receptors, Fibroblast Growth Factor / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Signal Transduction / physiology*
Transgenes
Grant Support
ID/Acronym/Agency:
T32 GM007499/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/EGL-15 protein, C elegans; 0/Egl-17 protein, C elegans; 0/Intercellular Signaling Peptides and Proteins; 0/Receptors, Fibroblast Growth Factor; 0/Recombinant Fusion Proteins; 62031-54-3/Fibroblast Growth Factors; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/VRK-1 protein, C elegans
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