Document Detail


Protein kinase C-like immunoreactivity in rod bipolar cells of the rat retina: a developmental study.
MedLine Citation:
PMID:  2069897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the retina of a variety of vertebrate species, a monoclonal antibody against protein kinase C (PKC) has been shown to label preferentially bipolar cells. Although the functional consequences of PKC activation in these cells is yet to be revealed, the present study was motivated in part by the possibility that the antibody might be used as a selective marker for examining the development of bipolar cells in the rat retina. Here, the developmental pattern and the dynamic changes of retinal cells expressing PKC-like immunoreactivity (PKC-LI) were studied and analyzed throughout postnatal life until adulthood. Upon its initial detection by immunohistochemistry on postnatal day (PD)-10, faint PKC-LI was limited to the central region of the retina, labeling cell bodies located at the scleral margin of the inner nuclear layer (INL) adjacent to the outer plexiform layer (OPL). On subsequent days, PKC-LI spread progressively to the peripheral retina and axon terminal bulbs at the vitreal margin of the inner plexiform layer (IPL) began showing the first signs of immunoreactive labeling. Not until PD-15, the time of eye opening, did PKC-LI in these cells increase to the extent such that their thin axons were immunoreactive. Each of these axons traversed the entire thickness of the IPL and divided into two or three short branches before ending as enlarged terminal bulbs. The morphology and the location of PKC-LI cells in both the developing and adult retina observed in our study are consistent with them being rod bipolar cells. By the end of the fourth postnatal week, the rod bipolar cells appeared mature, resembling those found in the adult.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
D R Zhang; H H Yeh
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Visual neuroscience     Volume:  6     ISSN:  0952-5238     ISO Abbreviation:  Vis. Neurosci.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-08-22     Completed Date:  1991-08-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8809466     Medline TA:  Vis Neurosci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  429-37     Citation Subset:  IM    
Affiliation:
Department of Neurobiology and Anatomy, University of Rochester Medical Center, NY 14642.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Axons / enzymology
Dark Adaptation
Female
Immunoenzyme Techniques
Light
Neurons / enzymology*
Photoreceptor Cells / enzymology*
Protein Kinase C / metabolism*
Rats
Rats, Inbred Strains
Retina / enzymology,  growth & development
Grant Support
ID/Acronym/Agency:
NS01340/NS/NINDS NIH HHS; NS24830/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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