Document Detail


Protein kinase C gamma expression mimics phorbol ester-induced transcriptional activation of a murine VL30 enhancer element.
MedLine Citation:
PMID:  2007096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein kinase C (PKC), a critical component in the regulation of cell growth, is thought to participate in transmitting the signals of certain cell surface receptor activation events to the nucleus. We have previously shown that stable expression of the PKC gamma isoenzyme in NIH 3T3 cells causes altered growth and enhanced tumorigenicity. In this report, we show that transient expression of the PKC gamma isoenzyme can trans-activate a murine VL30 enhancer element in a pattern similar to that of the phorbol ester tumor promoter 12-O-tetradecanoylphorbol-13-acetate. In contrast, ras activation of this element is distinct both quantitatively and qualitatively from PKC gamma and 12-O-tetradecanoylphorbol-13-acetate activation. These results provide direct evidence that PKC is the cellular mediator in the activation of phorbol ester-responsive genes and suggest a mechanism by which abnormal PKC expression might lead to altered growth control by changing the pattern of cellular gene expression.
Authors:
D A Persons; R D Owen; M C Ostrowski; O J Finn
Related Documents :
12709546 - Fcgamma receptor expression levels on monocytes are elevated in rheumatoid arthritis pa...
19116136 - Transcription factor 8 activates r-ras to regulate angiogenesis.
10843986 - Requirement of ras/mapk pathway activation by transforming growth factor beta for trans...
20090846 - Analysis of k-ras nuclear expression in fibroblasts and mesangial cells.
15121876 - Effect of mifepristone on the expression of cytokines in the human fallopian tube.
16583136 - The spen homolog msx2-interacting nuclear target protein interacts with the e2 ubiquiti...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  2     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-05-01     Completed Date:  1991-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-14     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Division / drug effects
Enhancer Elements, Genetic / drug effects,  genetics*
Gene Expression Regulation, Neoplastic / drug effects,  physiology*
Genes, ras / genetics
Isoenzymes / biosynthesis,  physiology
Luciferases / genetics
Mice
Molecular Sequence Data
Protein Kinase C / biosynthesis,  physiology*
Tetradecanoylphorbol Acetate / pharmacology*
Transcription, Genetic / drug effects,  genetics*
Transcriptional Activation / physiology
Transfection / genetics
Grant Support
ID/Acronym/Agency:
R01-AI-26935/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; 16561-29-8/Tetradecanoylphorbol Acetate; EC 1.13.12.-/Luciferases; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression of the jun-B gene during induction of monocytic differentiation.
Next Document:  Anatomic spread of india ink in the human intercostal space with radiographic correlation.