Document Detail

Protein kinase C gamma expression mimics phorbol ester-induced transcriptional activation of a murine VL30 enhancer element.
MedLine Citation:
PMID:  2007096     Owner:  NLM     Status:  MEDLINE    
Protein kinase C (PKC), a critical component in the regulation of cell growth, is thought to participate in transmitting the signals of certain cell surface receptor activation events to the nucleus. We have previously shown that stable expression of the PKC gamma isoenzyme in NIH 3T3 cells causes altered growth and enhanced tumorigenicity. In this report, we show that transient expression of the PKC gamma isoenzyme can trans-activate a murine VL30 enhancer element in a pattern similar to that of the phorbol ester tumor promoter 12-O-tetradecanoylphorbol-13-acetate. In contrast, ras activation of this element is distinct both quantitatively and qualitatively from PKC gamma and 12-O-tetradecanoylphorbol-13-acetate activation. These results provide direct evidence that PKC is the cellular mediator in the activation of phorbol ester-responsive genes and suggest a mechanism by which abnormal PKC expression might lead to altered growth control by changing the pattern of cellular gene expression.
D A Persons; R D Owen; M C Ostrowski; O J Finn
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  2     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-05-01     Completed Date:  1991-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-14     Citation Subset:  IM    
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
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MeSH Terms
Base Sequence
Cell Division / drug effects
Enhancer Elements, Genetic / drug effects,  genetics*
Gene Expression Regulation, Neoplastic / drug effects,  physiology*
Genes, ras / genetics
Isoenzymes / biosynthesis,  physiology
Luciferases / genetics
Molecular Sequence Data
Protein Kinase C / biosynthesis,  physiology*
Tetradecanoylphorbol Acetate / pharmacology*
Transcription, Genetic / drug effects,  genetics*
Transcriptional Activation / physiology
Transfection / genetics
Grant Support
Reg. No./Substance:
0/Isoenzymes; 16561-29-8/Tetradecanoylphorbol Acetate; EC 1.13.12.-/Luciferases; EC Kinase C

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