| Protein kinase C does not regulate diacylglycerol metabolism in aortic smooth muscle cells. | |
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MedLine Citation:
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PMID: 2233706 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of a reduction in protein kinase C activity on the metabolism of exogenous [3H]diC8 by freshly isolated smooth muscle cells from rabbit aorta and cultured A10 smooth muscle cells was determined. The metabolism of [3H]diC8 by both smooth muscle cell preparations was predominantly by hydrolysis to yield monoC8 and glycerol (lipase pathway); very little radioactivity was incorporated into phospholipids. Diacylglycerol lipase activity measured in vitro with A10 cell homogenates was much greater than diacylglycerol kinase activity. The addition of the protein kinase C inhibitor H-7 to incubations of isolated aortic smooth muscle cells and cultured A10 cells had no significant effect on the metabolism of [3H]diC8. Protein kinase C activity in cultured A10 cells preincubated for 20 h with a phorbol ester was reduced to 14% of control as a consequence of down-regulation, but diC8 metabolism was not changed. Therefore, protein kinase C does not regulate the metabolism of diacylglycerols in aortic smooth muscle cells. |
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Authors:
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M Chuang; K R Dell; D L Severson |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 96 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-12-07 Completed Date: 1990-12-07 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 69-77 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Calgary, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Animals Aorta / drug effects, enzymology* Diglycerides / metabolism* Down-Regulation Isoquinolines / pharmacology Kinetics Lipase / metabolism Muscle, Smooth / drug effects, enzymology* Piperazines / pharmacology Protein Kinase C / physiology* Rabbits Tritium |
| Chemical | |
Reg. No./Substance:
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0/Diglycerides; 0/Isoquinolines; 0/Piperazines; 10028-17-8/Tritium; 1069-87-0/1,2-dioctanoylglycerol; 84477-87-2/1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; EC 2.7.11.13/Protein Kinase C; EC 3.1.1.3/Lipase |
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