Document Detail


Protein kinase C does not regulate diacylglycerol metabolism in aortic smooth muscle cells.
MedLine Citation:
PMID:  2233706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of a reduction in protein kinase C activity on the metabolism of exogenous [3H]diC8 by freshly isolated smooth muscle cells from rabbit aorta and cultured A10 smooth muscle cells was determined. The metabolism of [3H]diC8 by both smooth muscle cell preparations was predominantly by hydrolysis to yield monoC8 and glycerol (lipase pathway); very little radioactivity was incorporated into phospholipids. Diacylglycerol lipase activity measured in vitro with A10 cell homogenates was much greater than diacylglycerol kinase activity. The addition of the protein kinase C inhibitor H-7 to incubations of isolated aortic smooth muscle cells and cultured A10 cells had no significant effect on the metabolism of [3H]diC8. Protein kinase C activity in cultured A10 cells preincubated for 20 h with a phorbol ester was reduced to 14% of control as a consequence of down-regulation, but diC8 metabolism was not changed. Therefore, protein kinase C does not regulate the metabolism of diacylglycerols in aortic smooth muscle cells.
Authors:
M Chuang; K R Dell; D L Severson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  96     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-12-07     Completed Date:  1990-12-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-77     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Calgary, Alberta, Canada.
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MeSH Terms
Descriptor/Qualifier:
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Animals
Aorta / drug effects,  enzymology*
Diglycerides / metabolism*
Down-Regulation
Isoquinolines / pharmacology
Kinetics
Lipase / metabolism
Muscle, Smooth / drug effects,  enzymology*
Piperazines / pharmacology
Protein Kinase C / physiology*
Rabbits
Tritium
Chemical
Reg. No./Substance:
0/Diglycerides; 0/Isoquinolines; 0/Piperazines; 10028-17-8/Tritium; 1069-87-0/1,2-dioctanoylglycerol; 84477-87-2/1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; EC 2.7.11.13/Protein Kinase C; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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